Zhang Yihan, Liu Yunxiu, Li Hailan, Sun Ting, Xu Min, Xu Pei
Key Laboratory of Specialty Agri-product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Sciences, China Jiliang University, Hangzhou, 310018, People's Republic of China.
Biochem Genet. 2024 Dec;62(6):4817-4836. doi: 10.1007/s10528-023-10658-5. Epub 2024 Feb 17.
The global prevalence of iron deficiency-induced "hidden hunger" highlights a critical health concern, underscoring the pressing need to improve iron nutrition through safe and efficient means, such as increasing iron intake from plant-based foods. Yellow Stripe-Like (YSL) genes play a crucial role in long-distance iron transport between source and sink tissues in plants. Here, we report on the analysis of YSL family genes in the common bean (Phaseolus vulgaris L.), an iron-rich legume crop. We identified 9 YSL genes in the common bean genome using BLAST and HMM methods. Gene duplication analysis revealed that PvYSL7a and PvYSL7b originated through tandem duplication events. Structural analysis noted an absence of conservative motifs in PvYSL3b and PvYSL7a, which led to distinct predicted 3D protein structures. Leveraging publicly available RNA-seq data from developing bean pods, the expression patterns of PvYSL genes alongside pod and seed development were analyzed. Notably, PvYSL7a and PvYSL7b, as well as PvYSL1a and PvYSL1b, exhibited diverged expression patterns in seeds, signifying their functional divergence in this tissue. Moreover, PvYSL3a and PvYSL3b exhibited divergent expression patterns in both pod walls and seeds during pod development, underscoring their distinct roles in facilitating iron transportation between pods and seeds. This study provides valuable insights into the gene regulatory basis of iron accumulation in bean pods and seeds.
缺铁引起的“隐性饥饿”在全球范围内普遍存在，这凸显了一个关键的健康问题，强调了通过安全有效的方式改善铁营养的迫切需求，比如增加从植物性食物中摄取铁。类黄条纹（YSL）基因在植物源库组织间的长距离铁运输中起着关键作用。在此，我们报告了对富含铁的豆科作物普通菜豆（Phaseolus vulgaris L.）中YSL家族基因的分析。我们使用BLAST和HMM方法在普通菜豆基因组中鉴定出9个YSL基因。基因复制分析表明，PvYSL7a和PvYSL7b起源于串联重复事件。结构分析指出，PvYSL3b和PvYSL7a中不存在保守基序，这导致了不同的预测三维蛋白质结构。利用发育中的豆荚的公开RNA测序数据，分析了PvYSL基因以及豆荚和种子发育过程中的表达模式。值得注意的是，PvYSL7a和PvYSL7b，以及PvYSL1a和PvYSL1b在种子中表现出不同的表达模式，表明它们在该组织中的功能存在差异。此外，PvYSL3a和PvYSL3b在豆荚发育过程中，在豆荚壁和种子中均表现出不同的表达模式，突出了它们在促进铁在豆荚和种子之间运输中的不同作用。这项研究为菜豆荚和种子中铁积累的基因调控基础提供了有价值的见解。
