Liu Huiling, Wang Da, Wang Zhenyu, Zhao Tong, Zhang Jingying, Wang Yan, Qiao Hongyu, Han Yuzhu
Modern Vegetable Industry Technology and Germplasm Resource Innovation Team, Northeast Asia Special Germplasm Resource Conservation and Innovation Center Vegetable Breeding Technology Innovation Team, College of Horticulture, Jilin Agricultural University, Changchun 130118, China.
Int J Mol Sci. 2024 Dec 5;25(23):13101. doi: 10.3390/ijms252313101.
The oil bean is a high-quality, economically valuable variety of kidney bean ( L.) that is widely cultivated in Northeast China. However, the prevalence of anthracnose, caused by a combination of factors, including continuous cropping over many years, has led to significant declines in both yield and quality. The mitogen-activated protein kinase (MAPK) cascade is a highly conserved plant cell signaling pathway that plays a pivotal role in plant growth and development, as well as responses to biotic stress. However, its role in the response of to anthracnose infection has not previously been reported. We identified and characterized thirteen genes () in the genome. These genes were found on eight of the eleven chromosomes of , and phylogenetic analyses classified them into four previously established subgroups (A-D). Analysis of the -acting elements in their promoter regions revealed the presence of multiple elements associated with light, hormone regulation, stress responses, and growth and development. An analysis of intraspecific collinearity revealed that whole-genome and/or segmental duplication, rather than tandem duplication, has been the primary driver of family expansion in . Transcriptome data revealed that the differed in their tissue-specific expression patterns, with showing particularly high expression in stems and stem tips and and showing relatively low expression across all tissues. In general, expression of the was higher in stems, stem tips, and pods than in other tissues and organs, suggesting that they may be particularly important for regulating stem and pod development. Analysis of the expression of in field-grown plants infected or uninfected with anthracnose revealed that the relative expression levels of , , , and exhibited particularly significant changes in response to anthracnose infection across different varieties, suggesting their potential involvement in the anthracnose response of Phaseolus vulgaris. This study reports the fundamental characteristics of the thirteen genes in , documents their expression patterns in diverse tissues, and offers preliminary insights into their responses to anthracnose infection, establishing a foundation for subsequent functional validation.
油豆角是菜豆（L.）的一个优质、具有经济价值的品种，在中国东北地区广泛种植。然而，由于包括多年连作在内的多种因素导致炭疽病盛行，致使产量和品质大幅下降。丝裂原活化蛋白激酶（MAPK）级联是一种高度保守的植物细胞信号通路，在植物生长发育以及对生物胁迫的响应中起关键作用。然而，其在菜豆对炭疽病感染的响应中的作用此前尚未见报道。我们在菜豆基因组中鉴定并表征了13个MAPK基因（）。这些基因分布在菜豆11条染色体中的8条上，系统发育分析将它们分为先前已确定的4个亚组（A - D）。对其启动子区域顺式作用元件的分析表明，存在多个与光、激素调节、胁迫响应以及生长发育相关的元件。种内共线性分析表明，全基因组和/或片段重复而非串联重复是菜豆MAPK家族扩张的主要驱动力。转录组数据显示，这些MAPK基因在组织特异性表达模式上存在差异，其中在茎和茎尖中表达特别高，而和在所有组织中的表达相对较低。总体而言，MAPK基因在茎、茎尖和豆荚中的表达高于其他组织和器官，表明它们可能对调节茎和豆荚发育尤为重要。对田间种植的感染或未感染炭疽病的植株中MAPK基因表达的分析表明，、、和的相对表达水平在不同品种中对炭疽病感染均表现出特别显著的变化，表明它们可能参与菜豆对炭疽病的响应。本研究报道了菜豆中13个MAPK基因的基本特征，记录了它们在不同组织中的表达模式，并对其对炭疽病感染的响应提供了初步见解，为后续功能验证奠定了基础。
