Xie R F
Zhonghua Zhong Liu Za Zhi. 1985 Nov;7(6):435-7.
This paper reports the effect of cyclophosphamide on the bone marrow hematopoiesis in the mouse. Cyclophosphamide 0.12 mg/g body weight was injected into the mice once and the observation lasted for 2 weeks. After the injection, peripheral leukocytes were reduced to the lowest level on day 4 and then increased higher than the control on day 7 to 14. The number of nucleated cell in the bone marrow was the lowest at the 48th hour and gradually became normal within two weeks. The pluripotent hemopoietic stem cells--CFU-S (colony forming unit-spleen) were depleted abruptly in 24 hours, then reproliferated exponentially to a peak on day 3, followed by a second decrease and came back to normal level on day 11 to 14. The changes of granulocytic progenitor cell CFU-D (colony forming unit-diffusion chamber) and CFU-C (colony forming unit-culture) were quite similar to that of CFU-S but their proliferation peak was on day 4. The peripheral leukocyte drop was slower and the return to normal was earlier than the hemopoietic cells. So the recovery of leukocyte count does not mean a real reconstruction of hematopoiesis. The bone marrow stroma observed by CFU-F (colony forming unit-fibroblastoid) assay and marrow microcirculation were also damaged and did not recover to normal during the observation. The bone marrow stroma and microcirculation showed a more serious damage.
本文报道了环磷酰胺对小鼠骨髓造血功能的影响。将0.12mg/g体重的环磷酰胺一次性注射到小鼠体内，观察持续2周。注射后，外周血白细胞在第4天降至最低水平，然后在第7天至14天升高至高于对照组水平。骨髓中有核细胞数量在第48小时最低，并在两周内逐渐恢复正常。多能造血干细胞——脾集落形成单位（CFU-S）在24小时内急剧减少，然后在第3天呈指数增殖至峰值，随后再次下降，并在第11天至14天恢复到正常水平。粒细胞祖细胞集落形成单位扩散盒（CFU-D）和集落形成单位培养（CFU-C）的变化与CFU-S相当相似，但其增殖峰值在第4天。外周血白细胞下降较慢，恢复正常比造血细胞早。因此，白细胞计数的恢复并不意味着造血功能的真正重建。通过成纤维细胞样集落形成单位（CFU-F）测定观察到的骨髓基质和骨髓微循环也受到损害，在观察期间未恢复正常。骨髓基质和微循环显示出更严重的损伤。
