Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Alameda Barros Terra S/N, São Domingos, Niterói, RJ 24020-150, Brazil.
Laboratório de Hepatites Virais, Instituto Oswaldo Cruz and Instituto de Tecnologia em Imunobiológicos, FIOCRUZ, Av. Brasil 4365, Manguinhos, Rio de Janeiro, RJ 210360-040, Brazil.
Res Vet Sci. 2024 Apr;170:105186. doi: 10.1016/j.rvsc.2024.105186. Epub 2024 Feb 13.
Feline parvovirus (FPV) and canine parvovirus (CPV) are over 98% identical in their DNA sequences, and the new variants of CPV (2a/2b/2c) have gained the ability to infect and replicate in cats. The aim of this study was to determine the genetic diversity in the VP2 gene of parvovirus strains circulating in domestic cats in Brazil during a 10-year period (2008-2017). For parvovirus screening, specific PCR was performed, and 25 (34.7%) of 72 cats tested positive. The PCR-positive samples were further subjected to full-length VP2 sequencing (1755 bp), and eight sequences (36%) were characterized as FPV, seven (28%) as CPV-2a and (32%) nine (36%) as CPV-2b. One sequence (RJ1085/11) showing typical CPV amino acid (aa) at residues 80 R, 93 N, 103 A, 232 I, and 323 N could not be characterized at this time. The sequences in this study displayed aa changes previously described for FPV (A14T, A91S, I101T, N564S, and A568G) from cats and CPV-2a/2b (S297N and Y324L) from dogs. However, the Y324L mutation has not yet been reported in any CPV-2a/2b strains from cats. Phylogenetic analysis supported the division of these sequences into two well-defined clades, clade 1 for FPV and clade 2 for CPV2a/2b. Unusually, the sequence RJ1085/11 was grouped separately. Two recombination breakpoints were detected by Bootscan and 3Seq methods implemented in the RDP4. This study is the first report of CPV-2a/2b in cats in Brazil. The detection of FPV strains with mutations characteristic of CPV indicates that Brazilian FPV strains have undergone genetic changes.
猫细小病毒（FPV）和犬细小病毒（CPV）在 DNA 序列上超过 98%相同，而 CPV 的新变体（2a/2b/2c）已获得感染和在猫体内复制的能力。本研究旨在确定巴西家猫在 10 年内（2008-2017 年）流行的细小病毒株的 VP2 基因遗传多样性。为了进行细小病毒筛查，进行了特异性 PCR，其中 72 只猫中有 25 只（34.7%）检测呈阳性。对 PCR 阳性样本进行全长 VP2 测序（1755 bp），其中 8 个序列（36%）被鉴定为 FPV，7 个（28%）为 CPV-2a，9 个（36%）为 CPV-2b。一个序列（RJ1085/11）在 80R、93N、103A、232I 和 323N 残基处显示典型 CPV 氨基酸（aa），目前无法鉴定。本研究中的序列显示 aa 变化，这些变化先前在猫的 FPV（A14T、A91S、I101T、N564S 和 A568G）和犬的 CPV-2a/2b（S297N 和 Y324L）中已有描述。然而，Y324L 突变尚未在任何 CPV-2a/2b 猫株中报道。系统进化分析支持这些序列分为两个明确的分支，分支 1 为 FPV，分支 2 为 CPV2a/2b。不同寻常的是，序列 RJ1085/11 被单独分组。通过 Bootscan 和 3Seq 方法在 RDP4 中检测到两个重组断点。本研究是巴西猫中 CPV-2a/2b 的首次报告。FPV 株中检测到与 CPV 特征性突变表明巴西 FPV 株发生了遗传变化。
