Chung Hee Chun, Kim Sung Jae, Nguyen Van Giap, Shin Sook, Kim Jae Young, Lim Suk Kyung, Park Yong Ho, Park BongKyun
Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.
Department of Veterinary Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi 100000, Vietnam.
J Vet Sci. 2020 May;21(3):e43. doi: 10.4142/jvs.2020.21.e43.
Canine parvovirus (CPV) and feline panleukopenia (FPV) cause severe intestinal disease and leukopenia.
In Korea, there have been a few studies on Korean FPV and CPV-2 strains. We attempted to investigate several genetic properties of FPV and CPV-2.
Several FPV and CPV sequences from around world were analyzed by Bayesian phylo-geographical analysis.
The parvoviruses strains were newly classified into FPV, CPV 2-I, CPV 2-II, and CPV 2-III genotypes. In the strains isolated in this study, Gigucheon, Rara and Jun belong to the FPV, while Rachi strain belong to CPV 2-III. With respect to CPV type 2, the new genotypes are inconsistent with the previous genotype classifications (CPV-2a, -2b, and -2c). The root of CPV-I strains were inferred to be originated from a USA strain, while the CPV-II and III were derived from Italy strains that originated in the USA. Based on VP2 protein analysis, CPV 2-I included CPV-2a-like isolates only, as differentiated by the change in residue S297A/N. Almost CPV-2a isolates were classified into CPV 2-III, and a large portion of CPV-2c isolates was classified into CPV 2-II. Two residue substitutions F267Y and Y324I of the VP2 protein were characterized in the isolates of CPV 2-III only.
We provided an updated insight on FPV and CPV-2 genotypes by molecular-based and our findings demonstrate the genetic characterization according to the new genotypes.
犬细小病毒（CPV）和猫泛白细胞减少症病毒（FPV）可引起严重的肠道疾病和白细胞减少症。
在韩国，针对韩国FPV和CPV-2毒株的研究较少。我们试图研究FPV和CPV-2的几种遗传特性。
通过贝叶斯系统发育地理学分析，对来自世界各地的几种FPV和CPV序列进行分析。
细小病毒毒株被重新分类为FPV、CPV 2-I、CPV 2-II和CPV 2-III基因型。在本研究分离出的毒株中，吉古川、拉拉和俊属于FPV，而拉奇毒株属于CPV 2-III。关于2型CPV，新基因型与先前的基因型分类（CPV-2a、-2b和-2c）不一致。推断CPV-I毒株的起源是一株美国毒株，而CPV-II和III则源自起源于美国的意大利毒株。基于VP2蛋白分析，CPV 2-I仅包括CPV-2a样分离株，通过残基S297A/N的变化进行区分。几乎所有CPV-2a分离株都被分类为CPV 2-III，并且大部分CPV-2c分离株被分类为CPV 2-II。VP2蛋白的两个残基取代F267Y和Y324I仅在CPV 2-III的分离株中被鉴定出来。
我们通过分子研究对FPV和CPV-2基因型提供了更新的见解，我们的研究结果展示了根据新基因型的遗传特征。
