Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih-796 015, Aizawl, Mizoram, India.
Department of Veterinary Medicine, West Bengal University of Animal & Fishery Sciences, Kolkata, West Bengal 700037, India.
Vet Ital. 2024 Jul 31;60(2). doi: 10.12834/VetIt.3430.23463.2.
The objective of the present study was to isolate and characterize the VP2 gene of parvoviruses from domestic cats in India. For that, 38 fecal samples were screened by PCR with 36.84% positivity. Sequence analysis of those isolates showed canine parvovirus type-2c (CPV-2c) as the predominant variant, followed by feline panleukopenia virus (FPV) and 2a. Phylogenetic analysis of the CPV-2c sequences revealed clustering with Singaporean, South Korean, Mongolian and Bangladeshi dog 2c sequences. Phylogenetic analysis of the 2a isolate (MZC 2) was found to be clustered with Indian, Thai and Singaporean dog 2a isolates. Similarly, all the four FPV sequences were ancestrally related to Indian dog and cat FPV sequences hinting towards interspecies transmission between dogs and cats. Both synonymous and non-synonymous mutations were evident in CPV-2c, 2a and FPV sequences indicative of active evolution. In cell culture medium, CPV-2 showed cytopathogenic effects at the third passage level. In conclusion, the study provided the first report of CPV-2c in cats from India, which demands for extensive epidemiological surveillance to monitor interspecies spread and to shed more light on viral phylogenomics, their distribution in the country and in the Southeast Asian region and usage of current vaccines.
本研究的目的是从印度的家猫中分离和鉴定细小病毒的 VP2 基因。为此，使用 PCR 对 38 份粪便样本进行了筛查，阳性率为 36.84%。对这些分离物的序列分析表明，犬细小病毒 2 型（CPV-2c）是主要的变异株，其次是猫泛白细胞减少症病毒（FPV）和 2a。CPV-2c 序列的系统进化分析显示与新加坡、韩国、蒙古和孟加拉国的犬 2c 序列聚类。MZC 2 分离株的 2a 序列的系统进化分析发现与印度、泰国和新加坡的犬 2a 分离株聚类。同样，所有四个 FPV 序列与印度犬和猫 FPV 序列在祖先上相关，提示犬和猫之间存在种间传播。CPV-2c、2a 和 FPV 序列中均存在同义和非同义突变，表明存在活跃的进化。在细胞培养物中，CPV-2 在第 3 代时表现出细胞病变效应。总之，本研究首次报道了印度猫中的 CPV-2c，这需要进行广泛的流行病学监测，以监测种间传播，并进一步了解病毒系统发生学、它们在该国和东南亚地区的分布以及现有疫苗的使用。
