Jilin Agricultural University, Changchun 130118, China.
Jilin Agriculture Science and Technology College, Jilin 132101, China.
Int J Biol Macromol. 2024 Apr;263(Pt 1):130226. doi: 10.1016/j.ijbiomac.2024.130226. Epub 2024 Feb 17.
With the improvement of modern living standards, the challenge of diabetic wound healing has significantly impacted the public health system. In this study, our objective was to enhance the bioactivity of taxifolin (TAX) by encapsulating it in liposomes using a thin film dispersion method. Additionally, polyvinyl alcohol/carboxymethyl chitosan-based hydrogels were prepared through repeated freeze-thawing. In vitro and in vivo experiments were conducted to investigate the properties of the hydrogel and its effectiveness in promoting wound healing in diabetic mice. The results of the experiments revealed that the encapsulation efficiency of taxifolin liposomes (TL) was 89.80 ± 4.10 %, with a drug loading capacity of 17.58 ± 2.04 %. Scanning electron microscopy analysis demonstrated that the prepared hydrogels possessed a porous structure, facilitating gas exchange and the absorption of wound exudates. Furthermore, the wound repair experiments in diabetic mice showed that the TL-loaded hydrogels (TL-Gels) could expedite wound healing by suppressing the inflammatory response and promoting the expression of autophagy-related proteins. Overall, this study highlights that TL-Gels effectively reduce wound healing time by modulating the inflammatory response and autophagy-related protein expression, thus offering promising prospects for the treatment of hard-to-heal wounds induced by diabetes.
随着现代生活水平的提高，糖尿病创面愈合的挑战对公共卫生系统产生了重大影响。在这项研究中，我们的目标是通过薄膜分散法将白杨素（TAX）包封在脂质体中以提高其生物活性。此外，还通过反复冻融制备了聚乙烯醇/羧甲基壳聚糖基水凝胶。进行了体外和体内实验来研究水凝胶的特性及其在促进糖尿病小鼠创面愈合方面的效果。实验结果表明，白杨素脂质体（TL）的包封效率为 89.80±4.10%，载药量为 17.58±2.04%。扫描电子显微镜分析表明，所制备的水凝胶具有多孔结构，有利于气体交换和伤口渗出物的吸收。此外，糖尿病小鼠的伤口修复实验表明，载 TL 的水凝胶（TL-Gels）能够通过抑制炎症反应和促进自噬相关蛋白的表达来加速伤口愈合。总的来说，这项研究表明 TL-Gels 通过调节炎症反应和自噬相关蛋白的表达有效地减少了伤口愈合时间，为治疗糖尿病引起的难愈性伤口提供了有前景的方法。
