College of traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, China.
College of traditional Chinese Medicine, Jilin Agriculture Science and Technology College, Jilin 132101, China.
Int J Biol Macromol. 2023 Jun 30;241:124537. doi: 10.1016/j.ijbiomac.2023.124537. Epub 2023 Apr 20.
In this study, a new wound dressing was developed to speed up the healing process of diabetic wounds. First of all, taxifolin liposome (TL) was manufactured in this study. Then, taxifolin (TAX) and TL were mixed with polyvinyl alcohol (PVA) and chitosan (CS) by electrostatic spinning to prepare nanocomposite membranes. Finally, the mechanism of nanocomposite membranes to accelerate diabetic wound healing was investigated. The diameter of TL-loaded polyvinyl alcohol/chitosan nanocomposite membranes (PVA/CS/TL) was 429.43 ± 78.07 nm. The results of in vitro experiments demonstrated that the PVA/CS/TL had better water absorption, water vapor transmission rate (WVTR), porosity, hydrophilicity, mechanical properties, slow-release, antioxidant capacity, and antibacterial properties. The results of in vivo experiments demonstrated that the wound healing rate of mice treated with PVA/CS/TL for eighteen days was 98.39 ± 0.34 %. Histopathological staining, immunohistochemical staining, and western blot experiments also demonstrated that PVA/CS/TL could promote wound healing in diabetic mice by inhibiting the activation of inhibitor kappa B alpha (IκBα)/nuclear factor-kappa B (NF-κB) signaling pathway and related pro-inflammatory factors to increase the expression of CD31 and VEGF in skin tissues. These results suggested that PVA/CS/TL could be a potential candidate for wound dressing to promote chronic skin wound healing.
在这项研究中，开发了一种新型的伤口敷料，以加速糖尿病伤口的愈合过程。首先，本研究制造了 taxifolin 脂质体（TL）。然后，将 taxifolin（TAX）和 TL 与聚乙烯醇（PVA）和壳聚糖（CS）混合通过静电纺丝制备纳米复合膜。最后，研究了纳米复合膜加速糖尿病伤口愈合的机制。载有 taxifolin 的聚乙烯醇/壳聚糖纳米复合膜（PVA/CS/TL）的直径为 429.43±78.07nm。体外实验结果表明，PVA/CS/TL 具有更好的吸水性、水蒸气透过率（WVTR）、孔隙率、亲水性、力学性能、缓释、抗氧化能力和抗菌性能。体内实验结果表明，用 PVA/CS/TL 处理 18 天的小鼠的伤口愈合率为 98.39±0.34%。组织病理学染色、免疫组织化学染色和 Western blot 实验也表明，PVA/CS/TL 可以通过抑制抑制剂 kappa B alpha（IκBα）/核因子 kappa B（NF-κB）信号通路和相关促炎因子的激活，增加皮肤组织中 CD31 和 VEGF 的表达，促进糖尿病小鼠的伤口愈合。这些结果表明，PVA/CS/TL 可能是一种有潜力的伤口敷料候选物，可促进慢性皮肤伤口愈合。
