Beijing Key Laboratory of Advanced Functional Polymer Composites, College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
Institute of Orthopedics, the Fourth Medical Center, Beijing Key Laboratory of Regenerative Medicine in Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
Int J Biol Macromol. 2024 Apr;263(Pt 1):130237. doi: 10.1016/j.ijbiomac.2024.130237. Epub 2024 Feb 17.
Breast cancer has become the most diagnosed cancer type, endangering the health of women. Patients with breast resection are likely to suffer serious physical and mental trauma. Therefore, breast reconstruction becomes an important means of postoperative patient rehabilitation. Polyvinyl alcohol hydrogel has great potential in adipose tissue engineering for breast reconstruction. However, its application is limited because of the lack of bioactive factors and poor structural stability. In this study, we prepared biodegradable polylactic acid-glycolic acid copolymer/polycaprolactone/gelatin (PPG) nanofibers. We then combined them with polyvinyl alcohol/collagen to create tissue engineering scaffolds to overcome limitations. We found that PPG fibers formed amide bonds with polyvinyl alcohol/collagen scaffolds. After chemical crosslinking, the number of amide bonds increased, leading to a significant improvement in their mechanical properties and thermal stability. The results showed that compared with pure PVA scaffolds, the maximum compressive stress of the scaffold doped with 0.9 g nanofibers increased by 500 %, and the stress loss rate decreased by 40.6 % after 10 cycles of compression. The presence of natural macromolecular gelatin and the changes in the pore structure caused by nanofibers provide cells with richer and more three-dimensional adsorption sites, allowing them to grow in three dimensions on the scaffold. So, the hydrogel scaffold by reinforcing polyvinyl alcohol hydrogel with PPG fibers is a promising breast reconstruction method.
乳腺癌已成为最常见的癌症类型，危害着女性的健康。接受乳房切除术的患者可能会遭受严重的身心创伤。因此，乳房重建成为术后患者康复的重要手段。聚乙烯醇水凝胶在乳房重建的脂肪组织工程中有很大的应用潜力。然而，由于缺乏生物活性因子和较差的结构稳定性，其应用受到限制。在本研究中，我们制备了可生物降解的聚乳酸-羟基乙酸共聚物/聚己内酯/明胶（PPG）纳米纤维。然后，我们将其与聚乙烯醇/胶原蛋白结合，以创建组织工程支架来克服这些限制。我们发现 PPG 纤维与聚乙烯醇/胶原蛋白支架形成酰胺键。经过化学交联后，酰胺键的数量增加，导致其机械性能和热稳定性显著提高。结果表明，与纯 PVA 支架相比，掺杂 0.9 g 纳米纤维的支架的最大压缩应力增加了 500%，在 10 次压缩循环后，其应力损失率降低了 40.6%。天然大分子明胶的存在以及纳米纤维引起的孔结构变化为细胞提供了更丰富和更三维的吸附位点，使它们能够在支架上进行三维生长。因此，通过 PPG 纤维增强聚乙烯醇水凝胶的水凝胶支架是一种有前途的乳房重建方法。
