School of Chemical and Petroleum Engineering, Shiraz University, Shiraz, Iran.
School of Chemical and Petroleum Engineering, Shiraz University, Shiraz, Iran.
Int J Biol Macromol. 2019 Jun 1;130:977-987. doi: 10.1016/j.ijbiomac.2019.03.045. Epub 2019 Mar 6.
The main objective of this work is to fabricate a nanofibrous scaffold to regenerate skin tissue. A scaffold composed of poly (vinyl alcohol)/gelatin/alginate was prepared using electrospinning method. To improve scaffold biocompatibility and wound healing properties, collagen, extracted from rat tail, was grafted on as-prepared nanofibers. The prepared scaffolds were characterized by SEM, FTIR, swelling ratio test, and water vapor transmission rate (WVTR) measurement. Cytotoxicity of the scaffolds against human fibroblasts and L929 (NCBI C161) cells were tested using direct and indirect methods, respectively. Fibroblast cell adhesion and proliferation on the scaffold were also investigated. Results of morphological studies showed that beadless nanofibers with 229 nm diameter were prepared. ATR-FTIR spectra of collagen grafted nanofiber mats confirmed presence of the collagen on their surface. Collagen grafted nanofibers showed higher swelling ratio than nanofibers without collagen graft. Collagen grafting decreased VWTR. Collagen grafting decreased both tensile strength and Young's modulus of the nanofibrous scaffolds while increased their elongation at break. MTT results showed that both scaffolds are biocompatible with higher cell viability for nanofibers with collagen grafting. Fibroblast cell culture on the scaffolds demonstrated that both of scaffolds have good cell viability and proliferation while collagen grafted scaffold showed better results.
这项工作的主要目的是制造一种用于皮肤组织再生的纳米纤维支架。采用静电纺丝法制备了由聚乙烯醇/明胶/海藻酸钠组成的支架。为了提高支架的生物相容性和伤口愈合性能，将从老鼠尾巴中提取的胶原蛋白接枝到所制备的纳米纤维上。通过 SEM、FTIR、溶胀比测试和水蒸气透过率（WVTR）测量对制备的支架进行了表征。分别采用直接法和间接法测试了支架对人成纤维细胞和 L929（NCBI C161）细胞的细胞毒性。还研究了支架上成纤维细胞的黏附和增殖情况。形态学研究结果表明，制备了直径为 229nm 的无珠纳米纤维。ATR-FTIR 光谱证实了胶原蛋白接枝纳米纤维垫表面存在胶原蛋白。与未接枝胶原蛋白的纳米纤维相比，接枝胶原蛋白的纳米纤维具有更高的溶胀比。胶原接枝降低了 VWTR。胶原接枝降低了纳米纤维支架的拉伸强度和杨氏模量，同时增加了其断裂伸长率。MTT 结果表明，两种支架均具有生物相容性，接枝胶原蛋白的纳米纤维具有更高的细胞活力。在支架上进行成纤维细胞培养表明，两种支架均具有良好的细胞活力和增殖能力，而接枝胶原蛋白的支架表现出更好的效果。
