Wu Di, Zhang Yanyan, Lin Nan, Wei Yicong
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian, China.
Sheng Wu Gong Cheng Xue Bao. 2024 Feb 25;40(2):542-561. doi: 10.13345/j.cjb.230371.
The leaves and roots of (thunb) nakai have different therapeutic effects in some clinical applications. In order to explore the tissue specific distribution differences of terpenoids in the leaves and roots of . , and to analyze the molecular mechanism of the formation of their pharmacodynamic quality differences. In this study, liquid chromatography-mass spectrometry (LC-MS) and Illumina HiSeq high-throughput sequencing techniques were respectively used to obtain the metabolome and transcriptome data of the leaves and roots of . . The metabolomics analysis showed that there were 50 differential terpenoids metabolites between the leaves and roots, including farnesylcysteine, d-glyceraldehyde 3-phosphate, and (R)-5-phosphomevalonate. The transcriptomics analysis indicated that there were 57 differentially expressed metabolic enzyme coding genes, including , , , , and . Moreover, there were seven transcription factors, including , , , which were predicted to participate in regulating the differences in terpenoid synthesis and accumulation between the leaves and roots of . . qRT-PCR results demonstrated that the expression changes of eight randomly selected enzyme genes involved in terpene synthesis between the leaves and roots of . , which were consistent with the transcriptome sequencing results. This study will help to elucidate the molecular mechanisms underlying the clinical efficacy differences between the leaves and roots of . , and facilitate the extraction, utilization, and resource development of . .
（文中植物名称未给出具体中文，暂保留英文）(thunb) nakai的叶和根在一些临床应用中有不同的治疗效果。为了探究（该植物）叶和根中萜类化合物的组织特异性分布差异，并分析其药效品质差异形成的分子机制。在本研究中，分别采用液相色谱 - 质谱联用（LC - MS）和Illumina HiSeq高通量测序技术获取了（该植物）叶和根的代谢组和转录组数据。代谢组学分析表明，叶和根之间有50种差异萜类代谢物，包括法尼基半胱氨酸、d - 甘油醛 - 3 - 磷酸和（R）-5 - 磷酸甲羟戊酸。转录组学分析表明，有57个差异表达的代谢酶编码基因，包括（此处基因名称未给出具体中文，暂保留英文）、（此处基因名称未给出具体中文，暂保留英文）、（此处基因名称未给出具体中文，暂保留英文）、（此处基因名称未给出具体中文，暂保留英文）和（此处基因名称未给出具体中文，暂保留英文）。此外，有7个转录因子，包括（此处转录因子名称未给出具体中文，暂保留英文）、（此处转录因子名称未给出具体中文，暂保留英文）、（此处转录因子名称未给出具体中文，暂保留英文），预测它们参与调节（该植物）叶和根之间萜类合成和积累的差异。qRT - PCR结果表明，随机选择的8个参与萜烯合成的酶基因在（该植物）叶和根之间的表达变化，与转录组测序结果一致。本研究将有助于阐明（该植物）叶和根临床疗效差异的分子机制，并促进（该植物）的提取、利用和资源开发。
