Wetzel D M, Haerter U L, Kelley D B
J Comp Physiol A. 1985 Dec;157(6):749-61. doi: 10.1007/BF01350072.
Vocalizations of South African clawed frogs are produced by contractions of laryngeal muscles innervated by motor neurons of the caudal medulla (within cranial nerve nucleus IX-X). We have traced afferents to laryngeal motor neurons in male and female frogs using retrograde axonal transport of horseradish peroxidase conjugated to wheat germ agglutinin (HRP-WGA). After iontophoretic injection of HRP-WGA into n. IX-X, retrogradely labelled neurons were seen in the contralateral n. IX-X, in rhombencephalic reticular nuclei, and in the pre-trigeminal nucleus of the dorsal tegmental area (DTAM) of both males and females.
南非爪蟾的发声是由延髓尾部（在第IX - X对脑神经核内）的运动神经元支配的喉肌收缩产生的。我们利用与小麦胚凝集素结合的辣根过氧化物酶（HRP - WGA）的逆行轴突运输，追踪了雄性和雌性青蛙中喉运动神经元的传入神经。在将HRP - WGA离子电渗注入第IX - X对脑神经核后，在雄性和雌性的对侧第IX - X对脑神经核、菱脑网状核以及背侧被盖区（DTAM）的三叉前核中均可见到逆行标记的神经元。
