Abdelaziz H, Mahran Abeer H, Elsewify T
Endodontic Department, Faculty of Dentistry, Ain Shams University, Cairo, Egypt.
Restorative Dental Sciences Department, College of Dentistry, Gulf Medical University, Ajman, United Arab Emirates.
Saudi Dent J. 2024 Jan;36(1):134-139. doi: 10.1016/j.sdentj.2023.10.018. Epub 2023 Oct 28.
The aim of this study was to evaluate the osteogenic differentiation ability and proliferation of apical papilla stem cells using nanoparticles of Neo MTA and bioactive glass.
Neo MTA and bioactive glass 45S5 nanoparticles were prepared and characterized using a transmission electron microscope and X-ray diffraction. Apical papilla stem cells were harvested from freshly-extracted fully-impacted wisdom teeth, cultured, and characterized using flow cytometric analysis. Tested nanomaterials were mixed and samples were classified into four equal groups as follows; Negative control group: SCAP with Dulbecco's modified eagle's medium, Positive control group: SCAP with inductive media, First experimental group: Neo MTA nanoparticles with SCAP, Second experimental group: Bioactive glass nanoparticles with SCAP. Osteoblastic differentiation was assessed using an alkaline phosphatase assay and RANKL expression using specific polyclonal antibodies by fluorescence microscope The proliferation of SCAP was assessed using cell count and viability of Trypan Blue in addition to an MTT assay.
Isolated SCAP showed a non-hematopoietic origin. Neo MTA showed the highest ALP concentration followed by bioactive glass nanoparticles, and negative control. Bioactive glass nanoparticles showed the highest H score for RANKL protein expression followed by Neo MTA, and negative control. Bioactive glass nanoparticles showed the highest viable cell count.
SCAP isolation is achievable from extracted fully impacted immature third molars. Both tested nanobiomaterials have the ability to induce osteogenic differentiation and proliferation of SCAP.
本研究旨在评估使用新型三氧化物多聚体（Neo MTA）纳米颗粒和生物活性玻璃对根尖乳头干细胞的成骨分化能力和增殖情况。
制备Neo MTA和生物活性玻璃45S5纳米颗粒，并使用透射电子显微镜和X射线衍射进行表征。从新鲜拔除的完全阻生智齿中获取根尖乳头干细胞，进行培养，并使用流式细胞术分析进行表征。将测试的纳米材料混合，样本分为四个相等的组，如下所示；阴性对照组：根尖乳头干细胞与杜尔贝科改良伊格尔培养基，阳性对照组：根尖乳头干细胞与诱导培养基，第一实验组：Neo MTA纳米颗粒与根尖乳头干细胞，第二实验组：生物活性玻璃纳米颗粒与根尖乳头干细胞。使用碱性磷酸酶测定法评估成骨细胞分化，并通过荧光显微镜使用特异性多克隆抗体评估RANKL表达。除了MTT测定法外，还使用细胞计数和台盼蓝活力评估根尖乳头干细胞的增殖。
分离出的根尖乳头干细胞显示非造血来源。Neo MTA显示碱性磷酸酶浓度最高，其次是生物活性玻璃纳米颗粒和阴性对照。生物活性玻璃纳米颗粒显示RANKL蛋白表达的H评分最高，其次是Neo MTA和阴性对照。生物活性玻璃纳米颗粒显示活细胞计数最高。
从拔除的完全阻生未成熟第三磨牙中可实现根尖乳头干细胞的分离。两种测试的纳米生物材料都有能力诱导根尖乳头干细胞的成骨分化和增殖。
