Oral Health Prev Dent. 2024 Feb 20;22:131-138. doi: 10.3290/j.ohpd.b4997059.
To assess the antioxidant and antineoplastic effects of Hibiscus sabdariffa Linn. on oral squamous cell carcinoma cells.
Human squamous cell carcinoma HSCC cells were tested for cytotoxicity by a methanol extract of Hibiscus sabdariffa (MEHSP). After 24, 48, and 72 h, the MTT assay and Trypan blue exclusion test were used to determine cell survival and death. 2, 2-diphenyl-1-picrylhydrazyl (DPPH), DNA Protection Assay (DPA), and ferric reducing antioxidant power assay (FRAPA) measured the antioxidant activity of MEHSP.
The antioxidant activity (%) ranged from 47.92-82.24 in the DPPH test, 11.61-73.65 in the DPA, and 4.97-52.09 in the FRAPA. The HSCC in-vitro cytotoxicity assay showed dose- and time-dependent cell viability. MEHSP at 5 μg/ml inhibited viable cells, while increasing MEHSP doses decreased cell viability. The Trypan blue exclusion test showed that MEHSP significantly reduced cell viability at 24, 48, and 72 h.
Hibiscus sabdariffa contains antioxidant and HSCC-cytotoxic properties.
评估洛神花（ Hibiscus sabdariffa Linn. ）对口腔鳞状细胞癌细胞的抗氧化和抗肿瘤作用。
用人源鳞状细胞癌细胞（HSCC）测试洛神花甲醇提取物（MEHSP）的细胞毒性。在 24、48 和 72 小时后，使用 MTT 测定法和台盼蓝排除试验来确定细胞存活和死亡。2,2-二苯基-1-苦肼基（DPPH）、DNA 保护试验（DPA）和铁还原抗氧化能力试验（FRAPA）测量 MEHSP 的抗氧化活性。
DPPH 试验中，抗氧化活性（%）范围为 47.92-82.24，DPA 为 11.61-73.65，FRAPA 为 4.97-52.09。HSCC 体外细胞毒性试验显示剂量和时间依赖性细胞活力。MEHSP 在 5 μg/ml 时抑制活细胞，而增加 MEHSP 剂量则降低细胞活力。台盼蓝排除试验表明，MEHSP 在 24、48 和 72 小时显著降低了细胞活力。
洛神花含有抗氧化和 HSCC 细胞毒性特性。
