Cheng Chen, Li Xin, Xu Gang, Lu Yanli, Low Sze Shin, Liu Guang, Zhu Long, Li Caidong, Liu Qingjun
Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, PR China.
Collaborative Innovation Center of TCM Health Management, Fujian University of Traditional Chinese Medicine, Fuzhou, 350108, PR China.
Biosens Bioelectron. 2021 Jan 15;172:112782. doi: 10.1016/j.bios.2020.112782. Epub 2020 Nov 1.
Wearable and flexible biosensing devices have been widely developed for in situ detections. Cortisol is a vital biomarker which plays crucial regulatory role in numerous physiological processes of the human body. Here, a wireless, battery-free, and flexible integrated patch is developed for real-time on-body sweat cortisol detection. The patch integrated with all-printed flexible electrochemical immunosensor, which was used to detect cortisol through differential pulse voltammetry (DPV). The near field communication (NFC) module on the patch enables wireless power harvesting and data interaction with an NFC-enabled smartphone, which makes the patch get rid of rigid batteries and realize epidermal on-body testing. Multiple in situ detections on volunteers' sweat on the surface of skin showed that the flexible integrated patch could reflect the circadian rhythm of the body's sweat cortisol level changes in relaxed mood or under stress, which could be confirmed with the enzyme linked immunosorbent assay (ELISA) kit. In this way, the patch provides a rapid-detecting, convenient, and non-invasive sensing solution for in situ analysis of sweat cortisol, which can be applied for the personalized mental health management.
可穿戴和柔性生物传感设备已被广泛开发用于原位检测。皮质醇是一种重要的生物标志物，在人体众多生理过程中发挥着关键的调节作用。在此，开发了一种用于实时检测人体汗液皮质醇的无线、无电池且柔性的集成贴片。该贴片集成了全印刷柔性电化学免疫传感器，用于通过差分脉冲伏安法（DPV）检测皮质醇。贴片上的近场通信（NFC）模块实现了无线能量收集以及与支持NFC的智能手机的数据交互，这使得贴片摆脱了刚性电池并实现了表皮人体测试。对志愿者皮肤表面汗液的多次原位检测表明，这种柔性集成贴片能够反映在放松情绪或压力状态下人体汗液皮质醇水平变化的昼夜节律，这可以通过酶联免疫吸附测定（ELISA）试剂盒得到证实。通过这种方式，该贴片为汗液皮质醇的原位分析提供了一种快速检测、方便且无创的传感解决方案，可应用于个性化心理健康管理。
