Future Industries Institute, STEM, University of South Australia, Mawson Lakes, SA 5095, Australia.
ARC Research Hub for Integrated Devices for End-User Analysis at Low-Levels (IDEAL), University of South Australia, Mawson Lakes, SA 5095, Australia.
ACS Appl Mater Interfaces. 2024 Mar 6;16(9):12042-12051. doi: 10.1021/acsami.3c17751. Epub 2024 Feb 21.
Rapid detection of pathogens and analytes at the point of care offers an opportunity for prompt patient management and public health control. This paper reports an open microfluidic platform coupled with active whispering gallery mode (WGM) microsphere resonators for the rapid detection of influenza viruses. The WGM microsphere resonators, precoated with influenza A polyclonal antibodies, are mechanically trapped in the open micropillar array, where the evaporation-driven flow continuously transports a small volume (∼μL) of sample to the resonators without auxiliaries. Selective chemical modification of the pillar array changes surface wettability and flow pattern, which enhances the detection sensitivity of the WGM resonator-based virus sensor. The optofluidic sensing platform is able to specifically detect influenza A viruses within 15 min using a few microliters of sample and displays a linear response to different virus concentrations.
在医疗现场快速检测病原体和分析物为及时的患者管理和公共卫生控制提供了机会。本文报道了一种开放式微流控平台，该平台与主动 whispering gallery mode (WGM) 微球谐振器耦合，用于快速检测流感病毒。WGM 微球谐振器预先涂有流感 A 多克隆抗体，被机械捕获在开放式微柱阵列中，在微柱阵列中，蒸发驱动的流动无需辅助即可连续将小体积（约 μL）的样品输送到谐振器。通过对柱阵列进行选择性化学修饰，可以改变表面润湿性和流动模式，从而增强基于 WGM 谐振器的病毒传感器的检测灵敏度。该光流控传感平台能够使用数微升样本在 15 分钟内特异性检测流感 A 病毒，并对不同病毒浓度呈线性响应。
