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采用带三重检测器(二极管阵列检测器-蒸发光散射检测器-质谱仪)的高效液相色谱法测定卡姆小麦中的游离和结合抗氧化剂。

Determination of free and bound antioxidants in Kamut® wheat by HPLC with triple detector (DAD-CAD-MS).

作者信息

Razem Mutasem, Morozova Ksenia, Ding Yubin, Ferrentino Giovanna, Scampicchio Matteo

机构信息

Faculty of Agricultural, Environmental and Food Sciences, Free University of Bozen-Bolzano, Piazza Università 1, 39100 Bolzano, Italy.

出版信息

Food Chem X. 2024 Feb 13;21:101216. doi: 10.1016/j.fochx.2024.101216. eCollection 2024 Mar 30.

DOI:10.1016/j.fochx.2024.101216
PMID:38384689
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10879663/
Abstract

Kamut® wheat ( ssp), an ancient, underutilized cereal, offers potential health benefits due to its phenolic compounds. This study aimed to investigate the antioxidant potential of Kamut® wheat's free and bound phenolic extracts using an HPLC system equipped with three detectors. The bound extracts, released after alkaline hydrolysis, exhibited higher total phenolic and flavonoid content compared to the free extracts ( < ). The total antioxidant capacity of bound extracts was six-fold greater than in free extracts ( < ). The main antioxidants in free extracts were tyrosine, phenylalanine, tryptophan, and apigenin. In bound extracts, ferulic acid, its dimers and trimer were present. Kamut® wheat exhibited a source of dietary antioxidants and should be considered a potential ingredient for the development of functional foods. Also, the HPLC-triple detector system is effective for in-depth profiling of antioxidant compounds, paving the way for future research on similar grains.

摘要

卡姆小麦(亚种)是一种古老且未得到充分利用的谷物,因其酚类化合物而具有潜在的健康益处。本研究旨在使用配备三种检测器的高效液相色谱系统,研究卡姆小麦游离和结合酚提取物的抗氧化潜力。碱性水解后释放的结合提取物与游离提取物相比,总酚和黄酮含量更高(<)。结合提取物的总抗氧化能力比游离提取物高六倍(<)。游离提取物中的主要抗氧化剂是酪氨酸、苯丙氨酸、色氨酸和芹菜素。结合提取物中存在阿魏酸及其二聚体和三聚体。卡姆小麦是膳食抗氧化剂的一个来源,应被视为开发功能性食品的潜在成分。此外,高效液相色谱 - 三重检测器系统对于抗氧化化合物的深入剖析是有效的,为未来对类似谷物的研究铺平了道路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5d1/10879663/e2f4cc105366/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5d1/10879663/32a10caf5168/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5d1/10879663/bb351dce9341/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5d1/10879663/3555ea846e13/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5d1/10879663/e2f4cc105366/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5d1/10879663/32a10caf5168/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5d1/10879663/bb351dce9341/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5d1/10879663/3555ea846e13/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5d1/10879663/e2f4cc105366/gr4.jpg

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J Physiol Sci. 2022 Aug 5;72(1):19. doi: 10.1186/s12576-022-00845-1.
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