Hayward M A, Barton M C, Shapiro D J
Mol Cell Endocrinol. 1985 Feb;39(2):91-8. doi: 10.1016/0303-7207(85)90124-8.
We have used plus-minus hybridization to identify Xenopus liver cDNA clones of mRNAs whose levels are regulated by estrogen. One clone identified in this way was shown to be a nearly full-length cDNA clone of the mRNA coding for a small 22 000 dalton estrogen-inducible serum protein (EISP). Quantitation of EISP mRNA levels by in vitro translation and by hybridization to the cloned DNA demonstrated a 7-12-fold estrogen induction of EISP mRNA, both in vivo and in primary Xenopus liver cultures. The kinetics of induction of EISP mRNA closely parallel those of the mRNA coding for the abundant estrogen-inducible serum protein, vitellogenin. In contrast, the massive, and toxic, estrogen-mediated accumulation of vitellogenin in serum of male Xenopus laevis is accompanied by a sharp decline in the levels of albumin mRNA and in the levels of the mRNAs coding for several other serum proteins.
我们利用正负杂交法来鉴定非洲爪蟾肝脏中那些mRNA水平受雌激素调控的cDNA克隆。通过这种方法鉴定出的一个克隆被证明是编码一种22000道尔顿的小的雌激素诱导血清蛋白（EISP）的mRNA的近乎全长的cDNA克隆。通过体外翻译以及与克隆DNA杂交对EISP mRNA水平进行定量分析，结果表明，在体内和原代非洲爪蟾肝脏培养物中，雌激素均可使EISP mRNA诱导7至12倍。EISP mRNA的诱导动力学与编码丰富的雌激素诱导血清蛋白卵黄生成素的mRNA的诱导动力学密切平行。相比之下，在雄性非洲爪蟾血清中，雌激素介导的大量且有毒的卵黄生成素积累伴随着白蛋白mRNA水平以及编码其他几种血清蛋白的mRNA水平的急剧下降。
