Schnitzerling H J, Nolan J
J Assoc Off Anal Chem. 1985 Jan-Feb;68(1):36-40.
A method has been developed for determining ivermectin in 5 mL samples of cattle blood by a 2-step process: cleanup solvent extraction followed by direct injection onto a normal phase liquid chromatography (LC) system with UV detection. Recovery was 77-80% +/- 5.5% standard deviation. Endogenous interference that may be present caused the lower limit of detection to be set at 4-5 ng/mL. The method was used to show that in blood the distribution of ivermectin favors plasma in a fixed proportion over cellular material, and further to provide a time-course profile of ivermectin in the whole blood of injected cattle. In whole blood, ivermectin concentration peaked between 3 and 5 days and dissipated slowly with a half-life of 3 days.
已开发出一种通过两步法测定5毫升牛血样本中伊维菌素的方法：首先进行净化溶剂萃取，然后直接注入配有紫外检测的正相液相色谱（LC）系统。回收率为77 - 80%，标准偏差为±5.5%。可能存在的内源性干扰导致检测下限设定为4 - 5纳克/毫升。该方法用于表明在血液中，伊维菌素在血浆中的分布比细胞物质更倾向于以固定比例存在，并进一步提供注射牛全血中伊维菌素的时间进程曲线。在全血中，伊维菌素浓度在3至5天达到峰值，并以3天的半衰期缓慢消散。
