State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China; College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan 450002, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
Cell Rep. 2024 Mar 26;43(3):113838. doi: 10.1016/j.celrep.2024.113838. Epub 2024 Feb 21.
Lysine acetylation is a dynamic post-translational modification of proteins. Extensive studies have revealed that the acetylation modulated by histone acetyltransferases and histone deacetylases (HDACs) plays a crucial role in regulating protein function. However, there has been limited focus on how HDACs regulate jasmonic acid (JA) biosynthesis in plants. Here, we uncover that the protein stability of OsLOX14, a critical enzyme involved in JA biosynthesis, is regulated by a histone deacetylase, OsHDA706, and is hindered by a viral protein. Our results show that OsHDA706 deacetylates OsLOX14 and enhances the stability of OsLOX14, leading to JA accumulation and an improved broad-spectrum rice antiviral defense. Furthermore, we found that the viral protein P2, encoded by the destructive rice stripe virus, disrupts the association of OsHDA706-OsLOX14, promoting viral infection. Overall, our findings reveal how HDAC manipulates the interplay of deacetylation and protein stability of a JA biosynthetic enzyme to enhance plant antiviral responses.
赖氨酸乙酰化是蛋白质的一种动态翻译后修饰。大量研究表明，组蛋白乙酰转移酶和组蛋白去乙酰化酶（HDACs）调节的乙酰化在调节蛋白质功能方面起着至关重要的作用。然而，HDACs 如何调节植物中茉莉酸（JA）的生物合成一直关注较少。在这里，我们发现参与 JA 生物合成的关键酶 OsLOX14 的蛋白稳定性受到一个组蛋白去乙酰化酶 OsHDA706 的调节，并且受到一个病毒蛋白的阻碍。我们的结果表明，OsHDA706 使 OsLOX14 去乙酰化，并增强 OsLOX14 的稳定性，导致 JA 积累和广谱水稻抗病毒防御能力提高。此外，我们发现由破坏性水稻条纹病毒编码的病毒蛋白 P2 破坏了 OsHDA706-OsLOX14 的结合，促进了病毒感染。总的来说，我们的发现揭示了 HDAC 如何操纵 JA 生物合成酶的去乙酰化和蛋白稳定性的相互作用，以增强植物抗病毒反应。
