Department of Biological Sciences, Columbia University, New York, NY, USA.
The Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
Science. 2024 Feb 23;383(6685):eadj7026. doi: 10.1126/science.adj7026.
In some mammals, notably humans, recombination occurs almost exclusively where the protein PRDM9 binds, whereas in vertebrates lacking an intact , such as birds and canids, recombination rates are elevated near promoter-like features. To determine whether PRDM9 directs recombination in nonmammalian vertebrates, we focused on an exemplar species with a single, intact ortholog, the corn snake (). Analyzing historical recombination rates along the genome and crossovers in pedigrees, we found evidence that PRDM9 specifies the location of recombination events, but we also detected a separable effect of promoter-like features. These findings reveal that the uses of PRDM9 and promoter-like features need not be mutually exclusive and instead reflect a tug-of-war that is more even in some species than others.
在一些哺乳动物中，尤其是人类中，重组几乎只发生在蛋白质 PRDM9 结合的地方，而在缺乏完整的哺乳动物中，如鸟类和犬科动物，重组率在类似于启动子的特征附近升高。为了确定 PRDM9 是否在非哺乳动物的脊椎动物中指导重组，我们专注于一个具有单个完整 直系同源物的典型物种，玉米蛇（）。通过分析基因组上的历史重组率和系谱中的交叉，我们发现有证据表明 PRDM9 指定了重组事件的位置，但我们也检测到类似于启动子的特征的可分离效应。这些发现表明，PRDM9 和类似于启动子的特征的用途不必相互排斥，而是反映了一种拔河比赛，在某些物种中比其他物种更为均衡。
