Kitamura Y, Shimada M, Go S, Matsuda H, Hatanaka K, Seki M
J Exp Med. 1979 Sep 19;150(3):482-90. doi: 10.1084/jem.150.3.482.
Two experimental systems were used to investigate the origin of precursor cells which differentiate into tissue mast cells in vivo. (a) Increase of mast cell number was examined in the skin, stomach, cecum, and mesentery of genetically mast cell-depleted WBB6F1 (WB X C57BL/6)-W/WV mice after the injection of various hematolymphoid cells of congenic +/+ mice. (b) Appearance of mast cells with giant granules was studied in irradiated C57BL/6-+/+ mice after the injection of lymphoid cells of C57BL/6-bgJ/bgJ (beige, Chediak-Higashi syndrome) mice. Concentrations of mast cell precursors in the thymus, lymph node and Peyer's patch were less than 0.1% of the concentration in the bone marrow. Neither treatment of donor bone marrow cells with anti-Thy-1.2 serum and complement nor thymectomy of the recipient mice affects the development of mast cells in the skin, stomach, cecum, and mesentery. Moreover, the number of mast cells increased to normal level when the skin of WBB6F1-W/WV mice was grafted on the back of nude athymic (BALB/c-nu/nu) mice. These results indicate that mast cell precursors are derived from hematopoietic tissues rather than lymphopoetic ones and that the differentiation of the precursor cells does not depend on T lymphocytes or the thymus.
使用了两种实验系统来研究在体内分化为组织肥大细胞的前体细胞的起源。(a) 在注射同基因 +/+ 小鼠的各种血液淋巴细胞后，检测基因性肥大细胞缺失的WBB6F1（WB×C57BL/6）-W/WV小鼠的皮肤、胃、盲肠和肠系膜中肥大细胞数量的增加情况。(b) 在注射C57BL/6-bgJ/bgJ（米色，切迪阿克-东综合征）小鼠的淋巴细胞后，研究受辐射的C57BL/6-+/+小鼠中出现巨大颗粒的肥大细胞。胸腺、淋巴结和派尔集合淋巴结中肥大细胞前体的浓度低于骨髓中浓度的0.1%。用抗Thy-1.2血清和补体处理供体骨髓细胞或对受体小鼠进行胸腺切除术均不影响皮肤、胃、盲肠和肠系膜中肥大细胞的发育。此外，将WBB6F1-W/WV小鼠的皮肤移植到裸胸腺缺失（BALB/c-nu/nu）小鼠的背部时，肥大细胞数量增加到正常水平。这些结果表明，肥大细胞前体来源于造血组织而非淋巴细胞组织，并且前体细胞的分化不依赖于T淋巴细胞或胸腺。
