Walter-Brendel-Centre of Experimental Medicine, University Hospital, Ludwig-Maximilians-University Munich, Munich, Germany.
Department of Cardiac Surgery, University Hospital, Ludwig-Maximilians-University Munich, Munich, Germany.
Commun Biol. 2024 Feb 22;7(1):220. doi: 10.1038/s42003-024-05886-3.
Dysfunctional Ca signaling affects the myocardial systole and diastole, may trigger arrhythmia and cause transcriptomic and proteomic modifications in heart failure. Thus, synchronous real-time measurement of Ca and force is essential to investigate the relationship between contractility and Ca signaling and the alteration of excitation-contraction coupling (ECC) in human failing myocardium. Here, we present a method for synchronized acquisition of intracellular Ca and contraction force in long-term cultivated slices of human failing myocardium. Synchronous time series of contraction force and intracellular Ca were used to calculate force-calcium loops and to analyze the dynamic alterations of ECC in response to various pacing frequencies, post-pause potentiation, high mechanical preload and pharmacological interventions in human failing myocardium. We provide an approach to simultaneously and repeatedly investigate alterations of contractility and Ca signals in long-term cultured myocardium, which will allow detecting the effects of electrophysiological or pharmacological interventions on human myocardial ECC.
功能失调的钙信号会影响心肌的收缩和舒张，可能引发心律失常，并导致心力衰竭中心的转录组和蛋白质组发生改变。因此，同步实时测量钙和力对于研究收缩性和钙信号之间的关系以及人类衰竭心肌中兴奋-收缩偶联（ECC）的改变至关重要。在这里，我们提出了一种在人类衰竭心肌的长期培养切片中同步获取细胞内钙和收缩力的方法。通过同步的收缩力和细胞内钙时间序列，计算力-钙环，并分析各种起搏频率、后暂停增强、高机械预载以及药物干预对人类衰竭心肌 ECC 的动态改变。我们提供了一种同时和重复研究长期培养心肌收缩性和钙信号改变的方法，这将有助于检测电生理或药物干预对人类心肌 ECC 的影响。
