• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Synthesis and functional activity of translation initiation regions in mRNA. 20-base polyribonucleotides from the replicase gene of phage MS2 and fr.

作者信息

Renhof R, Cielens I, Nikitina T, Sherinya L, Shomshtein Z, Gren E J

出版信息

FEBS Lett. 1985 Jun 17;185(2):277-81. doi: 10.1016/0014-5793(85)80922-4.

DOI:10.1016/0014-5793(85)80922-4
PMID:3838945
Abstract

Three 20-base polyribonucleotides, AAACAUGAGGAAUACCCAUG (I), AAACAUGAGGAAAACCCAUG (II), AAACAUGAAGAAUACCCAUG (III), corresponding to the minimal initiation region for the replicase gene of phage MS2 and fr or having some differences were synthesized using enzymatic methods. The template activity of the synthesized polynucleotides in initiation and their capacity to bind phage coat protein were studied under conditions optimal for native mRNA. Polynucleotides I and II exhibit template activity comparable to that of the native phage RNA fragments. Polynucleotide III with the destroyed SD sequence dit not manifest any functional activity either as template or in binding to MS2 phage coat protein.

摘要

相似文献

1
Synthesis and functional activity of translation initiation regions in mRNA. 20-base polyribonucleotides from the replicase gene of phage MS2 and fr.
FEBS Lett. 1985 Jun 17;185(2):277-81. doi: 10.1016/0014-5793(85)80922-4.
2
[Bacteriophage MS2 mutants with disrupted phage replicase repressor activity].[噬菌体复制酶阻遏活性被破坏的噬菌体MS2突变体]
Genetika. 1980;16(10):1719-28.
3
[Regulatory region of fr phage replicase cistron. II. Isolation and structure of specific fr RNA fragments].[噬菌体复制酶顺反子的调控区。II. 特异性fr RNA片段的分离与结构]
Mol Biol (Mosk). 1982 Sep-Oct;16(5):1109-15.
4
[Synthesis and functional activity of initiation regions of mRNA translation. II. RNA-ligase synthesis of hepta- and decaribonucleotides--components of 20-base polyribonucleotide templates].
Bioorg Khim. 1986 Apr;12(4):482-9.
5
[15-19-mer synthetic oligoribonucleotide as a model of the translation initiation segment of the phage fr replicase gene].
Bioorg Khim. 1995 Dec;21(12):920-4.
6
The regulatory region of MS2 phage RNA replicase cistron. Functional activity of individual MS2 RNA fragments.MS2噬菌体RNA复制酶顺反子的调控区域。单个MS2 RNA片段的功能活性。
J Mol Biol. 1978 Feb 15;119(1):101-31. doi: 10.1016/0022-2836(78)90272-3.
7
The regulatory region of MS2 phage RNA replicase cistron. IV. Functional activity of specific MS2 RNA fragments in formation of the 70 S initiation complex of protein biosynthesis.MS2噬菌体RNA复制酶顺反子的调控区。IV. 特定MS2 RNA片段在蛋白质生物合成70S起始复合物形成中的功能活性。
Nucleic Acids Res. 1979;6(5):1761-74. doi: 10.1093/nar/6.5.1761.
8
The regulatory region of MS2 phage RNA replicase cistron. III. Characterization of fragments resulting from S1 nuclease digestion.MS2噬菌体RNA复制酶顺反子的调控区域。III. S1核酸酶消化产生的片段的特性分析。
Nucleic Acids Res. 1979;6(5):1747-60. doi: 10.1093/nar/6.5.1747.
9
Mechanism of translational coupling between coat protein and replicase genes of RNA bacteriophage MS2.RNA噬菌体MS2外壳蛋白基因与复制酶基因之间的翻译偶联机制。
Nucleic Acids Res. 1985 Oct 11;13(19):6955-67. doi: 10.1093/nar/13.19.6955.
10
[Effect of synthetic polynucleotides and RNA on poly(C)-dependent poly(G) polymerase activity of Q beta replicase].[合成多核苷酸和RNA对Qβ复制酶的聚（C）依赖性聚（G）聚合酶活性的影响]
Biokhimiia. 1975 May-Jun;40(3):526-30.