Allan I, Pearce J H
J Gen Microbiol. 1979 May;112(1):53-9. doi: 10.1099/00221287-112-1-53.
Cell monolayer-grown chlamydiae (CGO) differed from egg-grown organisms (EGO) in their increased spontaneous infectivity relative to centrifuge-assisted infectivity for monolayers. For each population spontaneous: centrifuge-assisted infectivity ratios were constant over a wide dose range. Spontaneous infection increased linearly with time and could not be exhausted from either population by prolonged adsorption; there was no change in infectivity ratios in residual supernatants. Further, one passage of EGO through monolayers gave CGO with stable infectivity properties not increased by further cell passage yet reverting on a single passage in eggs. Spontaneous infection of monolayers with EGO gave progeny with the same infectivity ratios as monolayers infected with EGO by centrifugation. The change in properties following EGO infection of monolayers occurred prior to natural release from cells. We conclude that EGO and CGO are two phenotypically distinct, homogeneous populations. The two infection modes are not properties of subpopulations within EGO and CGO. The relationship of these observations on chlamydiae to other possible host-imposed phenomena is considered.
细胞单层培养的衣原体（CGO）与鸡胚培养的衣原体（EGO）不同，相对于单层细胞的离心辅助感染性，其自发感染性增加。对于每个群体，自发感染性与离心辅助感染性的比率在很宽的剂量范围内是恒定的。自发感染随时间呈线性增加，长时间吸附后，这两个群体的感染性都不会耗尽；残留上清液中的感染性比率没有变化。此外，EGO通过单层细胞传代一次后得到的CGO具有稳定的感染性特性，进一步通过细胞传代不会增加，但在鸡胚中传代一次就会恢复。用EGO自发感染单层细胞产生的子代与通过离心用EGO感染单层细胞产生的子代具有相同的感染性比率。EGO感染单层细胞后特性的变化发生在从细胞自然释放之前。我们得出结论，EGO和CGO是两个表型不同的同质群体。这两种感染模式不是EGO和CGO内亚群体的特性。文中还考虑了衣原体的这些观察结果与其他可能的宿主引发现象的关系。
