Cagliani L R, Consonni R
National Research Council, Institute of Chemical Sciences and Technologies "G. Natta" (SCITEC), Lab. NMR, V. Corti 12, 20133, Milan, Italy.
Heliyon. 2024 Feb 13;10(4):e25976. doi: 10.1016/j.heliyon.2024.e25976. eCollection 2024 Feb 29.
This study applied H NMR metabolomics to monitor the metabolite content of baked and seasoned zucchinis packaged in both compostable and plastic trays. Polar extracts of samples stored at 4 °C up to 35 days were investigated to check for metabolite changes upon shelf life. The evolution of the integral values of only the main metabolites responsible for sample differentiation (lactate, acetate, malate, α and β glucose and sucrose) were further analysed and compared. In particular, the evaluation of lactate and acetate amount, considered markers of fermentation progress, showed a comparable performance for the two types of packaging in preserving the freshness of seasoned zucchinis, confirming the maintenance of food product composition within the declared shelf life period in the recommended storage conditions. Albeit preliminary, the results support the NMR metabolomics as a tool for identifying candidate metabolites to monitor the shelf life of foods, thereby improving the understanding of molecular changes during storage.
本研究应用核磁共振氢谱代谢组学技术监测包装在可堆肥托盘和塑料托盘中的烘焙及调味西葫芦的代谢物含量。对在4℃下储存长达35天的样品的极性提取物进行了研究，以检查保质期内代谢物的变化。进一步分析并比较了仅负责样品区分的主要代谢物（乳酸、乙酸、苹果酸、α-葡萄糖、β-葡萄糖和蔗糖）积分值的变化。特别是，对乳酸和乙酸含量的评估（它们被视为发酵进程的标志物）表明，两种包装类型在保持调味西葫芦新鲜度方面具有相当的性能，证实了在推荐储存条件下食品成分在规定保质期内得以维持。尽管该研究尚属初步，但结果支持将核磁共振代谢组学作为一种识别候选代谢物以监测食品保质期的工具，从而增进对储存期间分子变化的理解。
