Ultrasound-assisted extraction (UAE) of Jerusalem artichoke tuber bio-active ingredient using optimized conditions of Box-Behnken response surface methodology.

The method of ultrasound-assisted extraction (UAE) was utilized to extract polyphenols from Jerusalem artichokes tuber (JAT). To determine the ideal values for ultrasound power (UP), extraction time (ET), and temperature (TP), a response surface methodology was utilized. JAT extracts were prepared using UAE and their content of total flavonoids (TFC), total polyphenols (TPC), ferric reducing-antioxidant activity (FRAP), and 2, 2'-diphenyl-1-picrylhydrazyl (DPPH) were determined. Accordingly, optimal settings were obtained where TP = 80.0 °C, ET = 14.99 min, and UP = 99.2 °C. These conditions caused TPC, TFC, FRAP, and % DPPH values to reach 4163.6 mg GAE/kg, 2731.6 mg RE/kg, 2.16 mmol/L, and 85.2% respectively, with general-desirability values of 1.00. In addition, DPPH (R = 0.950) and FRAP (R = 0.962) correlated with TPC, indicating that TPC contributed significantly to antioxidant activity. It was found that UAE extraction yields were higher than conventional extraction yields.