Afoakwah Newlove A, Tchabo William, Owusu-Ansah Patrick
Department of Food Science and Technology, Faculty of Agriculture, Food and Consumer Sciences, University for Development Studies, P. O. Box 1882, Tamale, Ghana.
Department of Food Science and Nutrition, National Advanced School of Agro-Industrial Sciences (ENSAI), University of Ngaoundere, Ngaoundere, Cameroon.
Heliyon. 2024 Feb 13;10(4):e25645. doi: 10.1016/j.heliyon.2024.e25645. eCollection 2024 Feb 29.
The method of ultrasound-assisted extraction (UAE) was utilized to extract polyphenols from Jerusalem artichokes tuber (JAT). To determine the ideal values for ultrasound power (UP), extraction time (ET), and temperature (TP), a response surface methodology was utilized. JAT extracts were prepared using UAE and their content of total flavonoids (TFC), total polyphenols (TPC), ferric reducing-antioxidant activity (FRAP), and 2, 2'-diphenyl-1-picrylhydrazyl (DPPH) were determined. Accordingly, optimal settings were obtained where TP = 80.0 °C, ET = 14.99 min, and UP = 99.2 °C. These conditions caused TPC, TFC, FRAP, and % DPPH values to reach 4163.6 mg GAE/kg, 2731.6 mg RE/kg, 2.16 mmol/L, and 85.2% respectively, with general-desirability values of 1.00. In addition, DPPH (R = 0.950) and FRAP (R = 0.962) correlated with TPC, indicating that TPC contributed significantly to antioxidant activity. It was found that UAE extraction yields were higher than conventional extraction yields.
采用超声辅助提取（UAE）法从菊芋块茎（JAT）中提取多酚。为了确定超声功率（UP）、提取时间（ET）和温度（TP）的理想值，采用了响应面法。使用UAE制备JAT提取物，并测定其总黄酮含量（TFC）、总多酚含量（TPC）、铁还原抗氧化能力（FRAP）和2,2'-二苯基-1-苦基肼（DPPH）。据此，获得了最佳设置，即TP = 80.0℃，ET = 14.99分钟，UP = 99.2℃。这些条件使TPC、TFC、FRAP和DPPH值分别达到4163.6 mg GAE/kg、2731.6 mg RE/kg、2.16 mmol/L和85.2%，综合可取性值为1.00。此外，DPPH（R = 0.950）和FRAP（R = 0.962）与TPC相关，表明TPC对抗氧化活性有显著贡献。结果发现，UAE提取率高于传统提取率。
