Li Feng, Raza Aun, Wang Yan-Wei, Xu Xiu-Quan, Chen Guan-Hua
Department of Food Science, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212001, Jiangsu Province, P. R. China.
Department of Cardiothoracic Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu Province, P. R. China.
Pharmacogn Mag. 2017 Jul-Sep;13(51):446-453. doi: 10.4103/pm.pm_159_16. Epub 2017 Jul 19.
Rattan tea is a medicinal plant that has been used for many years for the treatment of inflammation, fatty liver, tumor, diabetes, and hyperlipidemia.
A green and novel approach based on surfactant-mediated, ultrasonic-assisted extraction (SM-UAE) was developed for the extraction of antioxidant polyphenols from Rattan tea. A nonionic surfactant Tween-80 was selected as extraction solvent. The antioxidant activity was measured by total phenolic content (TPC) and ferric-reducing/antioxidant capacity (FRAC) assay.
Optimization of extraction parameters including concentration of solvent, ultrasonic time, and temperature were investigated by response surface methodology. The antioxidant activity was measured by TPC and FRAC assay.
The optimal extraction conditions were determined as 6.8% (v/v) of aqueous Tween-80, ultrasonic temperature of 54°C, and ultrasonic time of 28.8 min. Under these conditions, the highest TPC value of 360.4 mg gallic acid equivalent per gram of dry weight material (GAE/g DW) was recorded. Moreover, 6.8% (v/v) of aqueous Tween-80, ultrasonic temperature of 54.5°C, and ultrasonic time of 28.4 min were determined for the highest FRAC value of 478.2 μmol of Fe/g of weight material (μmol Fe/g DW). Compared with other methods, the TPC and FRAC values of 313.5 mg GAE/g DW and 389.6 μmol Fe/g DW were obtained by heat reflux extraction using ethanol as solvent, respectively, and 343.2 mg GAE/g DW and 450.1 μmol Fe/g DW were obtained by UAE using ethanol as solvent, respectively.
The application of SM-UAE markedly decreased extraction time or extraction cost and improved the extraction efficiency, compared with the other methods.
Surfactant-mediated ultrasonic-assisted extraction of antioxidant polyphenols from Response surface methodology used to optimize parameters and study combined effectsOptimized surfactant-mediated ultrasonic-assisted extraction process enhances the antioxidant phenolics extraction in less time. SM-UAE: Surfactant-mediated ultrasonic-assisted extraction; TPC: total phenolic content; FRAC: Ferric reducing antioxidant capacity; RSM: Response surface methodology; BBD: Box-Behnken design.
藤茶是一种药用植物，多年来一直用于治疗炎症、脂肪肝、肿瘤、糖尿病和高脂血症。
开发一种基于表面活性剂介导的超声辅助提取（SM-UAE）的绿色新颖方法，用于从藤茶中提取抗氧化多酚。选择非离子表面活性剂吐温80作为提取溶剂。通过总酚含量（TPC）和铁还原/抗氧化能力（FRAC）测定法测量抗氧化活性。
采用响应面法研究提取参数，包括溶剂浓度、超声时间和温度的优化。通过TPC和FRAC测定法测量抗氧化活性。
确定最佳提取条件为吐温80水溶液浓度6.8%（v/v）、超声温度54℃、超声时间28.8分钟。在此条件下，记录到最高TPC值为每克干重材料360.4毫克没食子酸当量（GAE/g DW）。此外，确定吐温80水溶液浓度6.8%（v/v）、超声温度54.5℃、超声时间28.4分钟时，FRAC值最高，为每克材料478.2微摩尔铁（μmol Fe/g DW）。与其他方法相比，以乙醇为溶剂采用热回流提取分别得到TPC值313.5 mg GAE/g DW和FRAC值389.6 μmol Fe/g DW，以乙醇为溶剂采用超声辅助提取分别得到343.2 mg GAE/g DW和450.1 μmol Fe/g DW。
与其他方法相比，SM-UAE的应用显著缩短了提取时间或降低了提取成本，并提高了提取效率。
表面活性剂介导的超声辅助从藤茶中提取抗氧化多酚响应面法用于优化参数和研究联合效应优化的表面活性剂介导的超声辅助提取工艺在更短时间内提高了抗氧化酚类物质的提取率。SM-UAE：表面活性剂介导的超声辅助提取；TPC：总酚含量；FRAC：铁还原抗氧化能力；RSM：响应面法；BBD：Box-Behnken设计
