Hossain Mohammad Amzad, AL-Raqmi Khulood Ahmed Salim, AL-Mijizy Zawan Hamood, Weli Afaf Mohammed, Al-Riyami Qasim
School of Pharmacy, College of Pharmacy and Nursing, University of Nizwa, P. O. Box 33, Postal Code 616, Nizwa, Sultanate of Oman.
Asian Pac J Trop Biomed. 2013 Sep;3(9):705-10. doi: 10.1016/S2221-1691(13)60142-2.
To prepare various crude extracts using different polarities of solvent and to quantitatively evaluate their total phenol, flavonoids contents and phytochemical screening of Thymus vulgaris collected from Al Jabal Al Akhdar, Nizwa, Sultanate of Oman.
The leave sample was extracted with methanol and evaporated. Then it was defatted with water and extracted with different polarities organic solvents with increasing polarities. The prepare hexane, chloroform, ethyl acetate, butanol and methanol crude extracts were used for their evaluation of total phenol, flavonoids contents and phytochemical screening study. The established conventional methods were used for quantitative determination of total phenol, flavonoids contents and phytochemical screening.
Phytochemical screening for various crude extracts were tested and shown positive result for flavonoids, saponins and steroids compounds. The result for total phenol content was the highest in butanol and the lowest in methanol crude extract whereas the total flavonoids contents was the highest in methanol and the lowest hexane crude extract.
The crude extracts from locally grown Thymus vulgaris showed high concentration of flavonoids and it could be used as antibiotics for different curable and uncurable diseases.
使用不同极性的溶剂制备各种粗提物，并对从阿曼苏丹国尼兹瓦的贾巴尔·阿克达尔采集的百里香的总酚、黄酮含量进行定量评估以及进行植物化学筛选。
将叶片样品用甲醇提取并蒸发。然后用水脱脂，再用极性递增的不同极性有机溶剂提取。制备的己烷、氯仿、乙酸乙酯、丁醇和甲醇粗提物用于总酚、黄酮含量评估和植物化学筛选研究。采用既定的常规方法对总酚、黄酮含量进行定量测定以及进行植物化学筛选。
对各种粗提物进行植物化学筛选，结果表明黄酮类、皂苷类和甾体类化合物呈阳性。总酚含量在丁醇粗提物中最高，在甲醇粗提物中最低，而总黄酮含量在甲醇粗提物中最高，在己烷粗提物中最低。
当地种植的百里香粗提物显示出高浓度的黄酮类化合物，可作为治疗不同可治愈和不可治愈疾病的抗生素。
