Hainan Engineering Research Center of Aquatic Resources Efficient Utilization in South China Sea, Key Laboratory of Seafood Processing of Haikou, School of Food Science and Engineering, Hainan University, Haikou 570228, China.
Key Laboratory of Tropical Fruits and Vegetables Quality and Safety for State Market Regulation, Hainan Institute for Food Control, Haikou 570314, China.
Biosensors (Basel). 2024 Jan 24;14(2):62. doi: 10.3390/bios14020062.
Ethephon (ETH), a commonly employed growth regulator, poses potential health risks due to its residue in fruits and vegetables, leading to both acute and subchronic toxicity. However, the detection accuracy of ETH is compromised by the color effects of the samples during the detection process. In this work, a multienzyme reaction-mediated electrochemical biosensor (MRMEC) was developed for the sensitive, rapid, and color-interference-resistant determination of ETH. Nanozymes FeO@Au-Pt and graphene nanocomplexes (GN-Au NPs) were prepared as catalysts and signal amplifiers for MRMEC. Acetylcholinesterase (AChE), acetylcholine (ACh), and choline oxidase (CHOx) form a cascade enzyme reaction to produce HO in an electrolytic cell. FeO@Au-Pt has excellent peroxidase-like activity and can catalyze the oxidation of 3,3',5,5'-tetramethvlbenzidine (TMB) in the presence of HO, resulting in a decrease in the characteristic peak current of TMB. Based on the inhibitory effect of ETH on AChE, the differential pulse voltammetry (DPV) current signal of TMB was used to detect ETH, offering the limit of detection (LOD) of 2.01 nmol L. The MRMEC method effectively analyzed ETH levels in mangoes, showing satisfactory precision (coefficient of variations, 2.88-15.97%) and recovery rate (92.18-110.72%). This biosensor holds promise for detecting various organophosphorus pesticides in food samples.
乙烯利（ETH）是一种常用的生长调节剂，由于其在水果和蔬菜中的残留，会导致急性和亚慢性毒性，对健康存在潜在风险。然而，在检测过程中，由于样品的颜色效应，ETH 的检测准确性受到影响。在这项工作中，开发了一种多酶反应介导的电化学生物传感器（MRMEC），用于灵敏、快速和抗颜色干扰测定 ETH。纳米酶 FeO@Au-Pt 和石墨烯纳米复合物（GN-Au NPs）被制备为 MRMEC 的催化剂和信号放大器。乙酰胆碱酯酶（AChE）、乙酰胆碱（ACh）和胆碱氧化酶（CHOx）在电解池中形成级联酶反应，产生 HO。FeO@Au-Pt 具有优异的过氧化物酶样活性，并且可以在 HO 的存在下催化 3,3',5,5'-四甲基联苯胺（TMB）的氧化，导致 TMB 的特征峰电流降低。基于 ETH 对 AChE 的抑制作用，使用差分脉冲伏安法（DPV）TMB 的电流信号来检测 ETH，检测限（LOD）为 2.01 nmol L。MRMEC 方法有效地分析了芒果中的 ETH 水平，显示出令人满意的精密度（变异系数，2.88-15.97%）和回收率（92.18-110.72%）。该生物传感器有望用于检测食品样品中的各种有机磷农药。
