Koung Ngeun Sai, Shimizu Miki, Kaneda Masahiro
Laboratory of Veterinary Diagnostic Imaging, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan.
Laboratory of Veterinary Anatomy, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan.
Biology (Basel). 2024 Jan 25;13(2):72. doi: 10.3390/biology13020072.
The myogenic differentiation potential of MSCs is a key factor in their potential use as a cell source for muscle tissue repair and regeneration. Additionally, evaluating the immunomodulatory properties of MSCs is important to highlight their potential for regulating inflammation and supporting tissue regeneration. Given the limited literature on muscle differentiation potential and immunomodulatory properties, this study aims to characterize rat ADP MSCs for treating muscle disease. We isolated MSCs from adipose tissues around the periscapular region of the rats. We used a monoculture method for the myogenic differentiation and modified the myogenic induction medium by supplementing it with the growth factors FGF, HGF, and IGF. In rat ADP MSCs, expression of the MSC-specific marker, CD90, was 87.7%, while CD44 was 42.8%. For genes involved in immunomodulation, and were highly expressed, while was poorly expressed. In addition to their trilineage differentiation potential, ADP MSCs exhibited the capacity to differentiate into myogenic cell lines, as evidenced by changes in cell morphology, leading to elongated and aligned structures and the expression of the MyoD and MYOG antibodies. The study found that ADP MSCs show great clinical promise for muscle regeneration.
间充质干细胞（MSCs）的成肌分化潜能是其作为肌肉组织修复和再生细胞来源的关键因素。此外，评估MSCs的免疫调节特性对于突出其调节炎症和支持组织再生的潜力很重要。鉴于关于肌肉分化潜能和免疫调节特性的文献有限，本研究旨在表征大鼠脂肪来源的间充质干细胞（ADP MSCs）用于治疗肌肉疾病的特性。我们从大鼠肩胛周围区域的脂肪组织中分离出MSCs。我们采用单培养方法进行成肌分化，并通过添加生长因子FGF、HGF和IGF来改良成肌诱导培养基。在大鼠ADP MSCs中，MSC特异性标志物CD90的表达率为87.7%，而CD44为42.8%。对于参与免疫调节的基因，[此处原文缺失具体基因名称]高度表达，而[此处原文缺失具体基因名称]表达较差。除了具有三系分化潜能外，ADP MSCs还表现出分化为成肌细胞系的能力，细胞形态的变化证明了这一点，导致细胞呈细长且排列整齐的结构，并表达MyoD和MYOG抗体。该研究发现，ADP MSCs在肌肉再生方面显示出巨大的临床应用前景。
