Department of Orthopaedics, Xuzhou No.1 People's Hospital, Xuzhou, China.
J Musculoskelet Neuronal Interact. 2022 Mar 1;22(1):123-131.
Mesenchymal stem cells (MSCs) have become seed cells and basic elements for bone regeneration and bone tissue engineering. The aim of the present study was to investigate the roles and mechanisms of bone morphogenetic protein 2 (BMP-2) on osteogenic differentiation of MSCs.
Primary MSCs were isolated from the femur and tibia bone of rats and then transfected with BMP-2 and PGC-1α adenovirus vectors. Alkaline phosphatase (ALP) activity and alizarin red staining were used to measure osteogenic differentiation of MSCs. Real-time PCR and western blot assays were performed to assess osteogenic differentiation-related proteins levels. The activities of mitochondrial respiratory chain complexes I and II and mitochondrial fluorescence intensity were used to explore mitochondria status during osteogenic differentiation of MSCs.
We found that the ability of BMP-2 overexpressed (OE) group osteogenic differentiation was significantly improved, compared with the negative control (NC) group. The results also indicated that BMP-2 can promote the activity of mitochondria. We further used the gain- and loss-of-function approaches to demonstrate that BMP-2 promotes mitochondrial activity by up-regulating PGC-1α to promote osteogenic differentiation of MSCs.
These results explored the important role of BMP-2 in the osteoblast differentiation of MSCs from a new perspective, providing a theoretical and experimental basis for bone defect and repair.
间充质干细胞（MSCs）已成为骨再生和骨组织工程的种子细胞和基本元素。本研究旨在探讨骨形态发生蛋白 2（BMP-2）对 MSCs 成骨分化的作用及其机制。
从大鼠股骨和胫骨中分离原代 MSCs，然后用 BMP-2 和 PGC-1α 腺病毒载体转染。碱性磷酸酶（ALP）活性和茜素红染色用于测量 MSCs 的成骨分化。实时 PCR 和 Western blot 分析用于评估成骨分化相关蛋白水平。线粒体呼吸链复合物 I 和 II 的活性和线粒体荧光强度用于研究 MSCs 成骨分化过程中线粒体状态。
我们发现，与阴性对照（NC）组相比，过表达 BMP-2（OE）组的成骨分化能力显著提高。结果还表明，BMP-2 可以促进线粒体的活性。我们进一步采用增益和失能方法证明，BMP-2 通过上调 PGC-1α 来促进 MSCs 的成骨分化，从而促进线粒体的活性。
这些结果从新的角度探讨了 BMP-2 在 MSCs 成骨分化中的重要作用，为骨缺损和修复提供了理论和实验依据。
