Raposo Francisco, Borja Rafael, Gutiérrez-González Julio A
Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (IG-CSIC), Campus Universitario Pablo de Olavide, Carretera de Utrera km 1, Edificio 46, 41013 Seville, Spain.
Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (IG-CSIC), Campus Universitario Pablo de Olavide, Carretera de Utrera km 1, Edificio 46, 41013 Seville, Spain.
Talanta. 2024 May 15;272:125771. doi: 10.1016/j.talanta.2024.125771. Epub 2024 Feb 15.
The Folin-Ciocalteu method can be considered to be the most widely used in laboratories around the world, to quantify the total polyphenols content. Many different variations found in this assay have been reported in the scientific literature. In this review, the full experimental conditions influencing the Folin-Ciocalteu assay have been comparatively assessed and discussed. Furthermore, few studies relating to the method validation have been evaluated according to the results of selectivity, linearity, precision, trueness, limit of determination, limit of quantification and robustness. In general, the results derived from the reviewed literature are widely variable according to both, the experimental factors selected and the performance parameters reported, making difficult the comparison of the overall results published.
福林-西奥尔特法可被视为全球实验室中用于定量总多酚含量最为广泛使用的方法。科学文献中已报道了该测定法存在的许多不同变体。在本综述中，对影响福林-西奥尔特测定法的完整实验条件进行了比较评估和讨论。此外，根据选择性、线性、精密度、准确性、测定限、定量限和稳健性的结果，对与该方法验证相关的少数研究进行了评估。总体而言，根据所选实验因素和所报告的性能参数，综述文献得出的结果差异很大，使得已发表的总体结果难以比较。
