Sheng Qi, Xu Qiuhua, Lan Zouran, Wu Zongfu
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210014, China.
Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing 210014, China.
Animals (Basel). 2024 Feb 8;14(4):572. doi: 10.3390/ani14040572.
is an important zoonotic pathogen that can cause meningitis and septicemia in swine and humans. Among numerous pathogenic serotypes, serotype 8 has distinctive characteristics such as a high detection rate and causing multi-host infection. There is no complete genome of serotype 8 strains so far. In this study, the complete genome of two serotype 8 strains, virulent strain 2018WUSS151 and non-virulent strain WUSS030, were sequenced. Comparative genomic analysis showed that the homology of the two genomes reaches 99.68%, and the main difference is the distinctive prophages. There are 83 genes unique to virulent strain 2018WUSS151, including three putative virulence-associated genes (PVGs). Two PVGs, and are passenger genes in ISSsu2 family transposons that are able to form circular DNA intermediates during transposition, indicating the possibility of horizontal transmission among strains. The deletion mutant of PVGs or attenuated the virulence of serotype 2 virulent SC070731 in a mouse infection model, confirming their role in virulence. These findings contribute to clarifying the genomic characterization of serotype 8 and pathogenesis.
是一种重要的人畜共患病原体，可导致猪和人类患脑膜炎和败血症。在众多致病血清型中，血清型8具有检测率高和引起多宿主感染等独特特征。目前尚无血清型8菌株的完整基因组。在本研究中，对两株血清型8菌株，即强毒株2018WUSS151和无毒株WUSS030进行了全基因组测序。比较基因组分析表明这两个基因组的同源性达到99.68%，主要差异在于独特的原噬菌体。强毒株2018WUSS151有83个独特基因，包括三个假定的毒力相关基因（PVGs）。两个PVGs， 和 是ISSsu2家族转座子中的过客基因，在转座过程中能够形成环状DNA中间体，表明在菌株间存在水平传播的可能性。在小鼠感染模型中，PVGs 或 的缺失突变体减弱了血清型2强毒株SC070731的毒力，证实了它们在毒力中的作用。这些发现有助于阐明血清型8的基因组特征和发病机制。
