Shi Jianzhou, Zhao Jinbing, Dong Bingxue, Li Na, Yao Lunguang, Sun Guirong
School of Life Science, Nanyang Normal University, Nanyang 473061, China.
The Shennong Laboratory, Zhengzhou 450046, China.
Animals (Basel). 2025 Aug 6;15(15):2300. doi: 10.3390/ani15152300.
The aim was to investigate the genetic effects of a SNP located in the precursor region of . (1) Single-nucleotide polymorphisms within precursor regions of microRNAs play crucial biological roles. (2) Utilizing a Gushi-Anka F resource population ( = 860), we screened and validated miRNA SNPs. A SNP mutation in the precursor region was identified. Specific primers were designed to amplify the polymorphic fragment. Genotyping was performed for this individual SNP across the population, using the MassArray system. Association analyses were conducted between this SNP and chicken growth and body measurement traits, carcass traits, meat quality traits, and serum enzyme activities. (3) The rs14098602 (+12 bp A > G) was identified within the precursor region of . Significant associations ( < 0.05) were observed between this SNP and chicken growth traits (body weight at the age of 0 day, body weight at the age of 2 weeks, and body weight at the age of 4 weeks), carcass traits (evisceration weight), meat quality traits (subcutaneous fat rate and pectoral muscle density), and serum enzyme activities (total protein, albumin, globulin, cholinesterase, and lactate dehydrogenase). (4) These findings suggest that the polymorphism at rs14098602 may influence chicken growth, meat quality, and serum biochemical indices, through specific mechanisms. The gene likely plays an important role in chicken development. Therefore, this SNP can serve as a molecular marker for genetic breeding and auxiliary selection of growth-related traits, facilitating the rapid establishment of elite chicken populations with superior genetic resources.
目的是研究位于……前体区域的一个单核苷酸多态性（SNP）的遗传效应。（1）微小RNA前体区域内的单核苷酸多态性发挥着关键的生物学作用。（2）利用固始-安卡F资源群体（n = 860），我们筛选并验证了miRNA的SNP。在……前体区域鉴定出一个SNP突变。设计了特异性引物来扩增多态性片段。使用MassArray系统对该群体中的这个个体SNP进行基因分型。对该SNP与鸡的生长和体尺性状、屠体性状、肉质性状以及血清酶活性进行了关联分析。（3）在……前体区域鉴定出rs14098602（+12 bp A > G）。观察到该SNP与鸡的生长性状（0日龄体重、2周龄体重和4周龄体重）、屠体性状（去内脏重）、肉质性状（皮下脂肪率和胸肌密度）以及血清酶活性（总蛋白、白蛋白、球蛋白、胆碱酯酶和乳酸脱氢酶）之间存在显著关联（P < 0.05）。（4）这些发现表明，rs14098602处的多态性可能通过特定机制影响鸡的生长、肉质和血清生化指标。……基因可能在鸡的发育中发挥重要作用。因此，这个SNP可作为生长相关性状遗传育种和辅助选择的分子标记，有助于快速建立具有优良遗传资源的优质鸡群体。
