Żarczyńska Katarzyna, Brym Paweł, Tobolski Dawid
Department and Clinic of Internal Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland.
Department of Animal Genetics, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland.
Animals (Basel). 2024 Feb 13;14(4):610. doi: 10.3390/ani14040610.
The transition period in high-yielding dairy cows is a critical phase marked by an elevated risk of oxidative stress. This study evaluated the effect of oral selenitetriglyceride supplementation on oxidative stress management in periparturient cows. A controlled experiment was conducted on 12 cows, divided into two groups: the experimental group (STG) received selenitetriglycerides (0.5 mg Se/kg BW), while the control group (CON) was given a placebo, starting 12 days before calving until the calving day. Blood and liver tissue samples were collected at predetermined intervals around the time of parturition. The study observed a significant increase in serum selenium levels and NEFA stabilization in the STG group compared with the control. Antioxidant parameters indicated elevated GSH-Px and CAT concentrations in the STG group. Liver gene expression analysis revealed a significant increase in SOD2 mRNA levels in the STG group (FC = 4.68, < 0.01). Conversely, GSH-Px3 expression significantly decreased (FC = 0.10, < 0.05) on the 7th day postpartum in the CON group. However, SOD1, SOD3, and CAT expressions remained stable in both groups. These findings highlight the beneficial role of selenitetriglycerides in enhancing antioxidant capacity and influencing specific gene expressions associated with oxidative stress management in dairy cows during the peripartum period.
高产奶牛的围产期是一个关键阶段，其特征是氧化应激风险升高。本研究评估了口服亚硒酸甘油酯对围产期奶牛氧化应激管理的影响。对12头奶牛进行了对照实验，分为两组：实验组（STG）在产犊前12天至产犊当天接受亚硒酸甘油酯（0.5 mg硒/千克体重），而对照组（CON）给予安慰剂。在分娩前后的预定时间间隔采集血液和肝脏组织样本。研究观察到，与对照组相比，STG组血清硒水平显著升高，非酯化脂肪酸（NEFA）稳定。抗氧化参数表明，STG组谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）浓度升高。肝脏基因表达分析显示，STG组超氧化物歧化酶2（SOD2）mRNA水平显著升高（FC = 4.68，P < 0.01）。相反，CON组产后第7天谷胱甘肽过氧化物酶3（GSH-Px3）表达显著降低（FC = 0.10，P < 0.05）。然而，两组中超氧化物歧化酶1（SOD1）、超氧化物歧化酶3（SOD3）和过氧化氢酶（CAT）的表达均保持稳定。这些发现突出了亚硒酸甘油酯在增强抗氧化能力以及影响围产期奶牛氧化应激管理相关特定基因表达方面的有益作用。
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