Żarczyńska Katarzyna, Sobiech Przemysław, Tobolski Dawid, Mee John F, Illek Josef
Department and Clinic of Internal Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury, 10-957, Olsztyn, Poland.
Animal and Bioscience Research Department, Teagasc, Moorepark Research Centre, Fermoy, Co, P61 C997, Cork, Ireland.
Ir Vet J. 2021 Apr 23;74(1):11. doi: 10.1186/s13620-021-00192-4.
Selenitetriglycerides are biologically active, organic forms of selenium formed as a result of the modification of selenic acid and sunflower oil. Studies in rats have shown that they are well absorbed and of low toxicity. There are no published studies on selenitetriglycerides supplementation in calves.
In this study, selenitetriglycerides were administered once orally on the 2nd day of life at a dose of 0.5 or 1 mg Se/kg body weight to each of six Holstein-Friesian calves while six control calves were not supplemented. Blood for determination of selenium concentration, glutathione peroxidase activity, haematological parameters, aspartate aminotransferase, creatine kinase, and lactate dehydrogenase activities and glucose, total protein, albumin, triglycerides, cholesterol, urea, and creatinine concentration was collected before supplementation (day 0) and 1, 2, 5, 10 and 14 days after supplementation. Selenitetriglycerides administration increased (P < 0.01) serum selenium concentration in supplemented calves as early as day1, from a mean of 63.4 to 184.22 µg/l in calves receiving selenium at a dose of 0.5 mg/kg BW, and from 63.17 to 200.33 µg/l in calves receiving 1 mg/kg. Serum selenium concentrations remained significantly higher compared to the control group throughout the experiment. Glutathione peroxidase activity was higher in supplemented than control calves, significantly so in animals receiving the 1 mg/kg dose of Se on the 10th and 14th days (P < 0.05). There were no significant differences in the haematological and biochemical parameters between the groups.
This experiment showed that supplementation with selenitetriglycerides could significantly improve blood selenium status in calves without adverse effects on haematological or biochemical parameters. These findings are essential prerequisites for future studies on selenitetriglycerides supplementation to manage clinical selenium deficiency in calves.
亚硒酸甘油酯是通过亚硒酸与葵花籽油改性形成的具有生物活性的有机硒形式。对大鼠的研究表明，它们易于吸收且毒性较低。目前尚无关于给犊牛补充亚硒酸甘油酯的已发表研究。
在本研究中，于出生后第2天对6头荷斯坦 - 弗里生犊牛每头口服一次亚硒酸甘油酯，剂量为0.5或1毫克硒/千克体重，而6头对照犊牛不进行补充。在补充前（第0天）以及补充后1、2、5、10和14天采集血液，用于测定硒浓度、谷胱甘肽过氧化物酶活性、血液学参数、天冬氨酸转氨酶、肌酸激酶和乳酸脱氢酶活性以及葡萄糖、总蛋白、白蛋白、甘油三酯、胆固醇、尿素和肌酐浓度。补充亚硒酸甘油酯后，早在第1天，补充组犊牛的血清硒浓度就有所升高（P < 0.01），接受0.5毫克/千克体重硒剂量的犊牛血清硒浓度从平均63.4微克/升升至184.22微克/升，接受1毫克/千克剂量的犊牛血清硒浓度从63.17微克/升升至200.33微克/升。在整个实验过程中，补充组的血清硒浓度始终显著高于对照组。补充组犊牛的谷胱甘肽过氧化物酶活性高于对照组，在接受1毫克/千克硒剂量的动物中，第10天和第14天显著升高（P < 0.05）。两组之间的血液学和生化参数无显著差异。
本实验表明，补充亚硒酸甘油酯可显著改善犊牛的血液硒状况，且对血液学或生化参数无不良影响。这些发现是未来研究补充亚硒酸甘油酯以解决犊牛临床硒缺乏问题的重要前提条件。
