Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Jilin 130102, P R China.
J Dairy Sci. 2013 Sep;96(9):5901-7. doi: 10.3168/jds.2013-6904. Epub 2013 Jul 17.
This study was conducted to evaluate the effects of chestnut tannins (CT) on performance and antioxidative status of transition dairy cows. Twenty multiparous Chinese Holstein cows in late gestation were paired according to expected calving date and randomly assigned either to a diet supplemented with CT (CNT, 10 g of CT/kg of diet, dry matter basis) or to an unsupplemented control (CON) diet from 3 wk prepartum to 3 wk postpartum. Blood samples were taken on d -21, 1, 7, and 21 relative to calving for analysis of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC), and malondialdehyde (MDA). Liver samples were taken by puncture biopsy on d 1 and 21 relative to calving for analysis of SOD, GSH-Px, and MDA. Data were analyzed for a completely randomized block design with repeated measures. The addition of CT had no significant effects on dry matter intake, body weight, body condition score, milk yield, 3.5% fat-corrected milk yield, and milk composition but did decrease milk MDA and somatic cell score in transition dairy cows. Dry matter intake decreased from d -21 to 0 and increased from d 1 to 21 relative to calving across treatments. During the experimental period, body weight and body condition score decreased, whereas milk MDA and somatic cell score increased across treatments. A time effect was also observed for plasma MDA, which peaked on d 1 relative to calving and remained higher than that on d -21 relative to calving across treatments. Addition of CT decreased MDA concentrations in plasma and liver. Neither time nor CT × time effects were observed for SOD and T-AOC in plasma and SOD and GSH-Px in liver; a time effect was observed for plasma GSH-Px, which peaked on d 1 relative to calving and remained higher than those on d -21 relative to calving across treatments. Addition of CT increased SOD, GSH-Px, and T-AOC activities in plasma and SOD and GSH-Px activities in liver. In conclusion, addition of CT might inhibit lipid peroxidation and increase antioxidant enzymes activities in plasma and liver of transition dairy cows. Supplementation of CT may be a feasible means to improve the antioxidative status of transition dairy cows.
本研究旨在评估板栗单宁（CT）对泌乳过渡期奶牛生产性能和抗氧化状态的影响。选择 20 头处于妊娠后期的中国荷斯坦奶牛，根据预计的分娩日期进行配对，并随机分为在产前 3 周至产后 3 周添加 CT（CNT，日粮中添加 10 g CT/kg 干物质）或不添加对照（CON）的日粮。在分娩前 21 天、1 天、7 天和 21 天采集血样，分析超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、总抗氧化能力（T-AOC）和丙二醛（MDA）。在分娩前 1 天和 21 天采集肝样，分析 SOD、GSH-Px 和 MDA。数据采用完全随机区组设计，重复测量。添加 CT 对干物质采食量、体重、体况评分、产奶量、3.5%校正乳产量和乳成分没有显著影响，但降低了泌乳过渡期奶牛的乳 MDA 和体细胞评分。干物质采食量从产前 21 天到 0 天下降，从 1 天到 21 天增加，不同处理组之间存在差异。在实验期间，体重和体况评分下降，而血浆 MDA 和体细胞评分增加。还观察到时间效应，血浆 MDA 在分娩前 1 天达到峰值，且高于不同处理组在分娩前 21 天的水平。添加 CT 降低了血浆和肝脏 MDA 浓度。在血浆 SOD 和 T-AOC 以及肝脏 SOD 和 GSH-Px 中未观察到时间和 CT×时间的影响；在血浆 GSH-Px 中观察到时间效应，其在分娩前 1 天达到峰值，并在整个处理组中高于在分娩前 21 天的水平。添加 CT 增加了血浆中 SOD、GSH-Px 和 T-AOC 的活性，以及肝脏中 SOD 和 GSH-Px 的活性。综上所述，添加 CT 可能抑制了泌乳过渡期奶牛的脂质过氧化作用，并提高了血浆和肝脏中抗氧化酶的活性。CT 的添加可能是改善泌乳过渡期奶牛抗氧化状态的一种可行方法。
