Iizuka T, Kanegasaki S, Makino R, Tanaka T, Ishimura Y
Biochem Biophys Res Commun. 1985 Jul 31;130(2):621-6. doi: 10.1016/0006-291x(85)90462-0.
Heterocyclic nitrogenous bases, such as pyridine and imidazole which bind to heme-iron in cytochromes, inhibited the respiratory burst in intact neutrophils and NADPH-dependent oxygen consumption in lysed cells. Inhibition was accompanied by a spectral change in reduced cytochrome b558 as judged by low-temperature spectroscopy at 77 K. The position and shape of the alpha-band of the cytochrome were significantly altered upon exposure to pyridine or some other bases. Both inhibition and spectral changes were reversible. The results are consistent with the view that cytochrome b558 is involved in the NADPH oxidase system in neutrophils.
杂环含氮碱基，如与细胞色素中的血红素铁结合的吡啶和咪唑，可抑制完整中性粒细胞的呼吸爆发以及裂解细胞中依赖烟酰胺腺嘌呤二核苷酸磷酸（NADPH）的氧消耗。通过77K低温光谱法判断，抑制作用伴随着还原型细胞色素b558的光谱变化。暴露于吡啶或其他一些碱基后，细胞色素的α带的位置和形状发生了显著改变。抑制作用和光谱变化都是可逆的。这些结果与细胞色素b558参与中性粒细胞NADPH氧化酶系统的观点一致。
