Departamento de Ecologia, Zoologia e Genética, Universidade Federal de Pelotas, Pelotas 96010-900, RS, Brazil.
Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos 13565-905, SP, Brazil.
Genes (Basel). 2024 Feb 19;15(2):258. doi: 10.3390/genes15020258.
(Charadriidae; Charadriiformes) comprises around 20 species commonly referred to as lapwings. In this study, by integrating cytogenetic and genomic approaches, we assessed the satellite DNA (satDNA) composition of one typical species, , with a highly conserved karyotype. We additionally underlined its role in the evolution, structure, and differentiation process of the present ZW sex chromosome system. Seven distinct satellite DNA families were identified within its genome, accumulating on the centromeres, microchromosomes, and the W chromosome. However, these identified satellite DNA families were not found in two other Charadriiformes members, namely and . The hybridization of microsatellite sequences revealed the presence of a few repetitive sequences in , with only two out of sixteen displaying positive hybridization signals. Overall, our results contribute to understanding the genomic organization and satDNA evolution in Charadriiform birds.
（Charadriidae; Charadriiformes）包括约 20 种通常被称为田凫的物种。在这项研究中，我们通过整合细胞遗传学和基因组学方法，评估了具有高度保守核型的典型物种 的卫星 DNA（satDNA）组成。我们还强调了它在当前 ZW 性染色体系统的进化、结构和分化过程中的作用。在其基因组中鉴定出了七个不同的卫星 DNA 家族，这些家族聚集在着丝粒、微染色体和 W 染色体上。然而，在另外两个 Charadriiformes 成员 和 中并未发现这些鉴定出的卫星 DNA 家族。微卫星序列的杂交揭示了 在 中存在一些重复序列，在 16 个序列中只有两个显示出阳性杂交信号。总的来说，我们的研究结果有助于理解 Charadriiform 鸟类的基因组组织和 satDNA 进化。
