Gómez-Vilarrubla Ariadna, Mas-Parés Berta, Carreras-Badosa Gemma, Bonmatí-Santané Alexandra, Martínez-Calcerrada Jose-Maria, Niubó-Pallàs Maria, de Zegher Francis, Ibáñez Lourdes, López-Bermejo Abel, Bassols Judit
Maternal-Fetal Metabolic Research Group, Girona Institute for Biomedical Research (IDIBGI), 17190 Salt, Spain.
Pediatric Endocrinology Research Group, Girona Institute for Biomedical Research (IDIBGI), 17190 Salt, Spain.
Biomedicines. 2024 Jan 27;12(2):301. doi: 10.3390/biomedicines12020301.
An epigenomic approach was used to study the impact of maternal pregestational body mass index (BMI) on the placenta and umbilical cord methylomes and their potential effect on the offspring's metabolic phenotype. DNA methylome was assessed in 24 paired placenta and umbilical cord samples. The differentially methylated CpGs associated with maternal pregestational BMI were identified and the metabolic pathways and the potentially related diseases affected by their annotated genes were determined. Two top differentially methylated CpGs were studied in 90 additional samples and the relationship with the offspring's metabolic phenotype was determined. The results showed that maternal pregestational BMI is associated with the methylation of genes involved in endocrine and developmental pathways with potential effects on type 2 diabetes and obesity. The methylation and expression of and genes in placenta and umbilical cord were related to several metabolic parameters in the offspring at 6 years (weight SDS, height SDS, BMI SDS, Δ BW-BMI SDS, FM SDS, waist, SBP, TG, HOMA-IR, perirenal fat; all < 0.05). Our data suggest that epigenetic analysis in placenta and umbilical cord may be useful for identifying individual vulnerability to later metabolic diseases.
采用表观基因组学方法研究孕前期母体体重指数（BMI）对胎盘和脐带甲基化组的影响及其对后代代谢表型的潜在作用。对24对胎盘和脐带样本进行了DNA甲基化组评估。鉴定出与孕前期母体BMI相关的差异甲基化CpG，并确定其注释基因所影响的代谢途径和潜在相关疾病。在另外90个样本中研究了两个高度差异甲基化的CpG，并确定了其与后代代谢表型的关系。结果表明，孕前期母体BMI与参与内分泌和发育途径的基因甲基化有关，对2型糖尿病和肥胖症可能产生影响。胎盘和脐带中 和 基因的甲基化和表达与后代6岁时的多个代谢参数相关（体重标准差评分、身高标准差评分、BMI标准差评分、体重变化-BMI标准差评分、脂肪量标准差评分、腰围、收缩压、甘油三酯、胰岛素抵抗指数、肾周脂肪；均P<0.05）。我们的数据表明，胎盘和脐带的表观遗传学分析可能有助于识别个体对后期代谢疾病的易感性。
