广泛性牙周炎：检测TAS2R16血清水平及常见基因多态性（rs860170、rs978739、rs1357949）
Generalised Periodontitis: Examining TAS2R16 Serum Levels and Common Gene Polymorphisms (rs860170, rs978739, rs1357949).
作者信息
Kriauciunas Albertas, Gedvilaite Greta, Bruzaite Akvile, Zekonis Gediminas, Razukevicius Dainius, Liutkeviciene Rasa
机构信息
Department of Prosthodontics, Lithuanian University of Health Sciences, Sukilėlių Str. 51, LT-50106 Kaunas, Lithuania.
Laboratory of Ophthalmology, Institute of Neuroscience, Medical Academy, Lithuanian University of Health Sciences, Eivenių Str. 2, LT-50009 Kaunas, Lithuania.
出版信息
Biomedicines. 2024 Jan 30;12(2):319. doi: 10.3390/biomedicines12020319.
The objective of this study was to evaluate and compare the associations between TAS2R16 serum levels and common gene rs860170, rs978739, and rs1357949 polymorphisms in patients affected by generalized periodontitis. The study enrolled 590 patients: 280 patients with periodontitis and 310 healthy controls as a reference group. Patients underwent periodontal examination and radiographic analysis to confirm the periodontitis diagnosis. Blood samples were collected, and the DNA salting-out method was used for DNA extraction from peripheral venous blood. Genotyping of (rs860170, rs978739, and rs1357949) was performed using real-time polymerase chain reaction (RT-PCR), and serum level analysis was performed for both periodontitis-affected patients and reference group subjects. The analysis of rs860170 (TT, CT, and CC) showed a statistically significant difference between generalized periodontitis and the reference group (41.8%, 58.2%, and 0% vs. 38.7%, 56.1%, and 5.2%, < 0.001). rs860170 (TT, CT, and CC) showed a statistically significant difference between males in generalized periodontitis and reference groups (38.4%, 61.6%, and 0% vs. 32.9%, 56.6%, and 10.5%, = 0.002). Female-specific analysis showed that the rs978739 C allele was more frequent in generalized periodontitis compared to the reference group (37.5% vs. 28.7%, = 0.016). Subjects aged 70 years and older demonstrated a statistically significant difference in rs860170 (TT, CT, and CC) between generalized periodontitis and the reference group (42.8%, 57.2%, and 0% vs. 38.6%, 53.8%, and 7.6%, = 0.003). TAS2R16 serum levels were elevated in generalized periodontitis compared to the reference group (0.112 (0.06) ng/mL vs. 0.075 (0.03) ng/mL, = 0.002). Females carrying the rs978739 C allele were more prone to generalized periodontitis development. Associations were found between rs860170 polymorphisms, elevated TAS2R16 serum levels, and generalized periodontitis development.
本研究的目的是评估和比较广泛性牙周炎患者血清中TAS2R16水平与常见基因rs860170、rs978739和rs1357949多态性之间的关联。该研究纳入了590名患者：280名牙周炎患者和310名健康对照作为参照组。患者接受了牙周检查和影像学分析以确诊牙周炎。采集血样，采用DNA盐析法从外周静脉血中提取DNA。使用实时聚合酶链反应（RT-PCR）对（rs860170、rs978739和rs1357949）进行基因分型，并对牙周炎患者和参照组受试者进行血清水平分析。rs860170（TT、CT和CC）分析显示，广泛性牙周炎组与参照组之间存在统计学显著差异（41.8%、58.2%和0% 对比 38.7%、56.1%和5.2%，P<0.001）。rs860170（TT、CT和CC）在广泛性牙周炎组男性与参照组男性之间存在统计学显著差异（38.4%、61.6%和0% 对比 32.9%、56.6%和10.5%，P = 0.002）。女性特异性分析显示，与参照组相比，rs978739 C等位基因在广泛性牙周炎患者中更为常见（37.5% 对比 28.7%，P = 0.016）。70岁及以上的受试者中，rs860170（TT、CT和CC）在广泛性牙周炎组与参照组之间存在统计学显著差异（42.8%、57.2%和0% 对比 38.6%、53.8%和7.6%，P = 0.003）。与参照组相比，广泛性牙周炎患者的TAS2R16血清水平升高（0.112（0.06）ng/mL 对比 0.075（0.03）ng/mL，P = 0.002）。携带rs978739 C等位基因的女性更易发生广泛性牙周炎。发现rs860170多态性、TAS2R16血清水平升高与广泛性牙周炎的发生之间存在关联。
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