探索味觉受体2型成员16（TAS2R16）蛋白及其单核苷酸变体在垂体腺瘤发生中的作用。

Exploring the Role of the TAS2R16 Protein and Its Single Nucleotide Variants in Pituitary Adenoma Development.

作者信息

Pileckaite Enrika, Vilkeviciute Alvita, Gedvilaite-Vaicechauskiene Greta, Kriauciuniene Loresa, Liutkeviciene Rasa

机构信息

Laboratory of Ophthalmology, Institute of Neuroscience, Lithuanian University of Health Sciences, LT-50161 Kaunas, Lithuania.

Department of Ophthalmology, Medical Academy, Lithuanian University of Health Sciences, LT-50161 Kaunas, Lithuania.

出版信息

Biomedicines. 2024 Sep 4;12(9):2022. doi: 10.3390/biomedicines12092022.

DOI:10.3390/biomedicines12092022

PMID:39335536

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11429365/

Abstract

BACKGROUND

Pituitary adenoma (PA) is a common benign tumor that develops in the pituitary gland, causing hormonal imbalances and potential health issues. The gene codes for a taste receptor and is involved in bitter taste perception, but there is currently no known direct link between this gene and pituitary adenoma.

METHODS

This study included 221 healthy controls and 131 patients with pituitary adenoma (PA) from the Lithuanian population. DNA was isolated from peripheral venous blood using the salt precipitation method. Genotyping was performed via RT-PCR. Statistical analysis was conducted with IBM SPSS Statistics 29.0 software, incorporating the Bonferroni correction for multiple comparisons.

RESULTS

This study found that the rs978739 C allele is less common in the non-invasive PA group compared to the control group ( = 0.045). The rs860170 CT genotype reduces the likelihood of developing non-invasive PA by 1.9-fold under the codominant ( = 0.024) and overdominant ( = 0.030) models. The odds of developing non-invasive PA are reduced by 2-fold under the dominant ( = 0.021) model for rs860170 CT + CC genotypes and by 2-fold under the additive ( = 0.018) model for each rs860170 C allele. The PA group had higher serum levels of TAS2R16 than the control group ( < 0.001). The present study found that patients with the rs978739 TT or CT genotype had higher serum TAS2R16 levels and protein concentrations than healthy individuals ( = 0.025 and = 0.019, respectively), and those with the AA or AG genotype of rs1357949 had higher protein concentrations ( = 0.005 and = 0.007, respectively).

CONCLUSIONS

The rs978739 C allele was less common in the non-invasive PA group compared to the control group, while the rs860170 CT genotype was linked to a reduced likelihood of developing non-invasive PA. Additionally, the PA group showed higher serum levels of TAS2R16, and increased serum protein concentrations were observed in PA patients with specific variants.

摘要

背景

垂体腺瘤（PA）是一种常见的良性肿瘤，发生于垂体，可导致激素失衡及潜在的健康问题。该基因编码一种味觉受体，参与苦味感知，但目前此基因与垂体腺瘤之间尚无已知的直接联系。

方法

本研究纳入了来自立陶宛人群的221名健康对照者和131例垂体腺瘤（PA）患者。采用盐沉淀法从外周静脉血中分离DNA。通过逆转录聚合酶链反应（RT-PCR）进行基因分型。使用IBM SPSS Statistics 29.0软件进行统计分析，并采用Bonferroni校正进行多重比较。

结果

本研究发现，与对照组相比，rs978739 C等位基因在非侵袭性PA组中更少见（P = 0.045）。在共显性模型（P = 0.024）和超显性模型（P = 0.030）下，rs860170 CT基因型使发生非侵袭性PA的可能性降低1.9倍。在rs860170 CT + CC基因型的显性模型（P = 0.021）下，发生非侵袭性PA的几率降低2倍，且在rs860170每个C等位基因的加性模型（P = 0.018）下降低2倍。PA组的血清TAS2R16水平高于对照组（P < 0.001）。本研究发现，rs978739 TT或CT基因型的患者血清TAS2R16水平和蛋白质浓度高于健康个体（分别为P = 0.025和P = 0.019），而rs1357949的AA或AG基因型患者的蛋白质浓度更高（分别为P = 0.005和P = 0.007）。