Lee Donghoon, Woo Chul-Woong, Heo Hwon, Ko Yousun, Jang Ji Sung, Na Seongwon, Kim Nari, Woo Dong-Cheol, Kim Kyung Won, Lee Do-Wan
Faculty of Health Sciences, Higher Colleges of Technology, Fujairah P.O. Box 1626, United Arab Emirates.
Convergence Medicine Research Center, Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea.
Biomedicines. 2024 Feb 7;12(2):384. doi: 10.3390/biomedicines12020384.
Chemical exchange saturation transfer with glutamate (GluCEST) imaging is a novel technique for the non-invasive detection and quantification of cerebral Glu levels in neuromolecular processes. Here we used GluCEST imaging and H magnetic resonance spectroscopy (H MRS) to assess in vivo changes in Glu signals within the hippocampus in a rat model of depression induced by a forced swim test. The forced swimming test (FST) group exhibited markedly reduced GluCEST-weighted levels and Glu concentrations when examined using H MRS in the hippocampal region compared to the control group (GluCEST-weighted levels: 3.67 ± 0.81% vs. 5.02 ± 0.44%, < 0.001; and Glu concentrations: 6.560 ± 0.292 μmol/g vs. 7.133 ± 0.397 μmol/g, = 0.001). Our results indicate that GluCEST imaging is a distinctive approach to detecting and monitoring Glu levels in a rat model of depression. Furthermore, the application of GluCEST imaging may provide a deeper insight into the neurochemical involvement of glutamate in various psychiatric disorders.
基于谷氨酸的化学交换饱和转移（GluCEST）成像技术是一种用于在神经分子过程中无创检测和定量脑内谷氨酸（Glu）水平的新技术。在此，我们使用GluCEST成像和氢磁共振波谱（1H MRS）来评估强迫游泳试验诱导的大鼠抑郁模型中海马体内Glu信号的变化。与对照组相比，强迫游泳试验（FST）组在海马区域使用1H MRS检测时，GluCEST加权水平和Glu浓度显著降低（GluCEST加权水平：3.67±0.81%对5.02±0.44%，P<0.001；Glu浓度：6.560±0.292μmol/g对7.133±0.397μmol/g，P = 0.001）。我们的结果表明，GluCEST成像对于检测和监测大鼠抑郁模型中的Glu水平是一种独特的方法。此外，GluCEST成像的应用可能会为谷氨酸在各种精神疾病中的神经化学作用提供更深入的见解。
