Abalymov Anatolii A, Anisimov Roman A, Demina Polina A, Kildisheva Veronika A, Kalinova Alexandra E, Serdobintsev Alexey A, Novikova Nadezhda G, Petrenko Dmitry B, Sadovnikov Alexandr V, Voronin Denis V, Lomova Maria V
Science Medical Centre, Saratov State University, 83 Astrakhanskayast, Saratov 410012, Russia.
Institute of Chemistry, Saratov State University, 83 Astrakhanskayast, Saratov 410012, Russia.
Biomedicines. 2024 Feb 16;12(2):443. doi: 10.3390/biomedicines12020443.
The effect of an extremely low frequency alternating magnetic field (ELF AMF) at frequencies of 17, 48, and 95 Hz at 100 mT on free and internalized 4T1 breast cancer cell submicron magnetic mineral carriers with an anticancer drug, mitoxantrone, was shown. The alternating magnetic field (100 mT; 17, 48, 95 Hz; time of treatment-10.5 min with a 30 s delay) does not lead to the significant destruction of carrier shells and release of mitoxantrone or bovine serum albumin from them according to the data of spectrophotometry, or the heating of carriers in the process of exposure to magnetic fields. The most optimal set of factors that would lead to the suppression of proliferation and survival of cells with anticancer drug carriers on the third day (in comparison with the control and first day) is exposure to an alternating magnetic field of 100 mT in a pulsed mode with a frequency of 95 Hz. The presence of magnetic nanocarriers in cell lines was carried out by a direct label-free method, space-resolved Brillouin light scattering (BLS) spectrometry, which was realized for the first time. The analysis of the series of integrated BLS spectra showed an increase in the magnetic phase in cells with a growth in the number of particles per cell (from 10 to 100) after their internalization. The safety of magnetic carriers in the release of their constituent ions has been evaluated using atomic absorption spectrometry.
研究表明了频率为17、48和95Hz、强度为100mT的极低频交变磁场（ELF AMF）对载有抗癌药物米托蒽醌的游离及内化4T1乳腺癌细胞亚微米磁性矿物载体的影响。根据分光光度法数据，交变磁场（100mT；17、48、95Hz；处理时间 - 10.5分钟，延迟30秒）不会导致载体外壳的显著破坏，也不会使米托蒽醌或牛血清白蛋白从载体中释放出来，并且在磁场暴露过程中载体也不会发热。与对照和第一天相比，在第三天能导致载有抗癌药物载体的细胞增殖和存活受到抑制的最优化因素组合是暴露于频率为95Hz的脉冲模式100mT交变磁场。通过直接免标记方法——空间分辨布里渊光散射（BLS）光谱法首次实现了对细胞系中磁性纳米载体的检测。对一系列积分BLS光谱的分析表明，细胞内化后，随着每个细胞内颗粒数量的增加（从10个增加到100个），细胞中的磁性相有所增加。已使用原子吸收光谱法评估了磁性载体在其组成离子释放方面的安全性。
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