Cao Chunna, Zhou Qian, Kang Yuxin, Akhatayeva Zhanerke, Liu Peiyao, Bai Yuheng, Li Ran, Jiang Yu, Zhang Qingfeng, Lan Xianyong, Pan Chuanying
Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Sheep Industry Development Center of Yulin City, Yulin, 719053, China.
Theriogenology. 2024 Apr 15;219:59-64. doi: 10.1016/j.theriogenology.2024.02.019. Epub 2024 Feb 20.
The BMPR1B gene is a major determinant of sheep reproductive capacity. Previous studies revealed that Q249R (FecB) is a profound variant of BMPR1B that influences the ovulation rate and litter size in sheep. However, unlike Q249R locus, the full spectrum of single nucleotide polymorphisms (SNPs) within BMPR1B has not been extensively studied. A systematic screen of SNPs in BMPR1B would facilitate the discovery of novel variants that are associated with litter size. This study aimed to investigate SNPs in the BMPR1B gene via whole genome sequence (WGS) data from 2409 individuals of 75 sheep breeds worldwide. Herein, a total of 9688 variants were screened, among which 15 were coding variants and 8 were novel changes. Specifically, we presented the most comprehensive frequency distribution map of the well-known FecB mutation to date. Besides, among the above-mentioned SNPs, one synonymous mutation (g.30050773C > T) was found to be likely under selection and is potentially associated with fecundity in Duolang sheep. Thus, our study greatly expands the variation repertoire of the ovine BMPR1B gene and provides a valuable resource for exploring causative mutations and genetic markers associated with litter size.
BMPR1B基因是绵羊繁殖能力的主要决定因素。先前的研究表明，Q249R（FecB）是BMPR1B的一个深刻变体，影响绵羊的排卵率和产仔数。然而，与Q249R位点不同，BMPR1B内单核苷酸多态性（SNP）的全谱尚未得到广泛研究。对BMPR1B中的SNP进行系统筛选将有助于发现与产仔数相关的新变体。本研究旨在通过来自全球75个绵羊品种的2409个个体的全基因组序列（WGS）数据，研究BMPR1B基因中的SNP。在此，共筛选出9688个变体，其中15个是编码变体，8个是新变化。具体而言，我们展示了迄今为止最全面的著名FecB突变频率分布图。此外，在上述SNP中，发现一个同义突变（g.30050773C>T）可能处于选择之下，并且可能与多浪羊的繁殖力相关。因此，我们的研究极大地扩展了绵羊BMPR1B基因的变异库，并为探索与产仔数相关的致病突变和遗传标记提供了宝贵资源。
