Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India.
Anim Biotechnol. 2011 Jul-Sep;22(3):151-62. doi: 10.1080/10495398.2011.589239.
The present study was designed for screening polymorphism of known fecundity genes in prolific Indian Bonpala sheep. Employing tetra-primer amplification refractory mutation system PCR, 11-point mutations of BMP1B, BMP15, and GDF9 genes of 97 Bonpala ewes were genotyped. The FecB locus of the BMPR1B gene and two loci (G1 and G4) of GDF9 gene were found to be polymorphic. In FecB locus, three genotypes, namely, wild type (Fec++, 0.02), heterozygous (FecB+, 0.23), and mutant (FecBB, 0.75) were detected. At G1 locus of GDF9 gene, three genotypes, namely, wild type (GG, 0.89), heterozygous (GA, 0.10), and mutant (AA, 0.01) were detected. At G4 locus of GDF9 gene, three genotypes, namely, wild type (AA, 0.01), heterozygous (AG, 0.14), and mutant (GG, 0.85) were detected. Statistically no significant correlation of polymorphism of FecB, G1, and G4 loci and litter size was found in this breed. All five loci of BMP15 and three loci of GDF 9 genes were monomorphic. This study reports Bonpala sheep as the first sheep breed where concurrent polymorphism at three important loci (FecB, G1, and G4) of two different fecundity genes (BMPR1B and GDF9) has been found.
本研究旨在筛选高产印度邦帕拉绵羊中已知繁殖力基因的多态性。采用四引物扩增受阻突变系统 PCR 技术，对 97 只邦帕拉母羊的 BMP1B、BMP15 和 GDF9 基因的 11 个点突变进行了基因分型。发现 BMPR1B 基因的 FecB 座和 GDF9 基因的两个座（G1 和 G4）存在多态性。在 FecB 座中，检测到三种基因型，即野生型（Fec++，0.02）、杂合型（FecB+，0.23）和突变型（FecBB，0.75）。在 GDF9 基因的 G1 座中，检测到三种基因型，即野生型（GG，0.89）、杂合型（GA，0.10）和突变型（AA，0.01）。在 GDF9 基因的 G4 座中，检测到三种基因型，即野生型（AA，0.01）、杂合型（AG，0.14）和突变型（GG，0.85）。统计分析表明，该品种 FecB、G1 和 G4 座的多态性与产仔数之间无显著相关性。BMP15 的所有五个座和 GDF9 基因的三个座均为单态性。本研究报道邦帕拉绵羊是第一个在两个不同繁殖力基因（BMPR1B 和 GDF9）的三个重要座（FecB、G1 和 G4）同时发现多态性的绵羊品种。
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