Mano Y, Sakai H, Komano T
J Virol. 1979 Jun;30(3):650-6. doi: 10.1128/JVI.30.3.650-656.1979.
phi X174am3trD, a temperature-resistant mutant of bacteriophage phi X174am3, exhibited a reduced ability to grow in a dnaP mutant, Escherichia coli KM107, at the restrictive temperature (43 degrees C). Under conditions at which the dnaP gene function was inactivated, the amount and the rate of phi X174am3trD DNA synthesis were reduced. The efficiency of phage attachment to E. coli KM107 at 43 degrees C was the same as to the parental strain, E. coli KD4301, but phage eclipse and phage DNA penetration were inhibited in E. coli KM107 at 43 degrees C. It is suggested that the dnaP gene product, which is necessary for the initiation of host DNA replication, participates in the conversion of attached phages to eclipsed particles and in phage DNA penetration in vivo in normal infection.
噬菌体φX174am3的耐温突变体φX174am3trD在限制温度（43摄氏度）下，于dnaP突变体大肠杆菌KM107中生长的能力有所下降。在dnaP基因功能失活的条件下，φX174am3trD DNA合成的量和速率均降低。43摄氏度时，噬菌体附着于大肠杆菌KM107的效率与附着于亲本菌株大肠杆菌KD4301的效率相同，但在43摄氏度时，大肠杆菌KM107中的噬菌体隐蔽期和噬菌体DNA穿透受到抑制。这表明，宿主DNA复制起始所必需的dnaP基因产物，在正常感染过程中参与了附着噬菌体向隐蔽颗粒的转化以及噬菌体DNA在体内的穿透。
