Wang Sheng-Lin, Zai Qing-Yong, Chen Hua-Guo, Li Jia-Wen, Zhou Xin
Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment,Guizhou Normal University Guiyang 550001, China Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine Guiyang 550001, China.
Zhongguo Zhong Yao Za Zhi. 2024 Jan;49(1):110-122. doi: 10.19540/j.cnki.cjcmm.20231025.301.
Studying the physicochemical properties and biological activities of Lycium barbarum polysaccharides(LBPs) is of great significance. The previous study had extracted LBPs(LBP-1, LBP-2, LBP-3, LBP-4, and LBP-5) by five different methods(cold water extraction, boiling water reflux extraction of the residue after cold water extraction, ultrasonic extraction with 50% ethanol, ultrasonic extraction with 25% ethanol of the residue after 50% ethanol extraction, and hot water extraction). In this study, the structures of the obtained five LBPs were characterized by UV spectroscopy, thermogravimetric analysis, and scanning electron microscopy. Furthermore, the antioxidant, blood lipid-lowering, nitrosation-inhibting, acetylcholinesterase-inhibiting, and tyrosinase-inhibiting activities of the five LBPs were measured in vitro. The results showed that high-temperature extraction destroyed the polysaccharide structure, while ultrasound-assisted extraction ensured the structural integrity. The thermal stability and degradation behaviors differed among the five LBPs. However, the UV spectroscopic results of the five LBPs did not show significant differences, and all of the five LBPs showed the characteristic absorption peaks of proteins. LBP-3 and LBP-4 exhibited strong antioxidant activity, while LBP-3 had the strongest blood lipid-lowering activity. In addition, LBP-3 outperformed other LBPs in inhibiting nitrosation and acetylcholineste-rase, and LBP-2 showed the strongest inhibitory effect on tyrosinase. This study explored the effects of different extraction methods on the physicochemical properties and biological activities of LBPs, with a view to providing a basis for the selection of suitable extraction methods to obtain LBPs with ideal biological activities.
研究枸杞多糖（LBPs）的理化性质和生物活性具有重要意义。先前的研究通过五种不同方法（冷水提取、冷水提取后残渣的沸水回流提取、50%乙醇超声提取、50%乙醇提取后残渣的25%乙醇超声提取以及热水提取）提取了LBPs（LBP-1、LBP-2、LBP-3、LBP-4和LBP-5）。在本研究中，通过紫外光谱、热重分析和扫描电子显微镜对获得的五种LBPs的结构进行了表征。此外，还对这五种LBPs的抗氧化、降血脂、抑制亚硝化、抑制乙酰胆碱酯酶和抑制酪氨酸酶活性进行了体外测定。结果表明，高温提取破坏了多糖结构，而超声辅助提取确保了结构完整性。五种LBPs的热稳定性和降解行为有所不同。然而，五种LBPs的紫外光谱结果没有显示出显著差异，并且所有五种LBPs都显示出蛋白质的特征吸收峰。LBP-3和LBP-4表现出较强的抗氧化活性，而LBP-3具有最强的降血脂活性。此外，LBP-3在抑制亚硝化和乙酰胆碱酯酶方面优于其他LBPs，LBP-2对酪氨酸酶的抑制作用最强。本研究探讨了不同提取方法对LBPs理化性质和生物活性的影响，以期为选择合适的提取方法以获得具有理想生物活性的LBPs提供依据。
