State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
College of Pharmacy, Chengdu University of Chinese Medicine, Chengdu, China; State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
J Pharm Biomed Anal. 2020 Jun 5;185:113219. doi: 10.1016/j.jpba.2020.113219. Epub 2020 Feb 29.
It has been proven that polysaccharides have bioactivities and are beneficial to cure many diseases. Lycium barbarum fruit is widely used as a functional food all over the world, which main active component is L. barbarum polysaccharides (LBPs). In this study, classical hot water extraction (HWE), microwave assisted extraction (MAE), ultrasonic assisted extraction (UAE) and pressurized liquid extraction (PLE) were used to extracted LBP. The chemical properties of LBPs were evaluated in terms of total polysaccharide contents, uronic acid contents and protein contents. High performance size exclusion chromatography coupled with multi angle laser light scattering and refractive index detector was applied to measure the characters such as molecular weight, radius of gyration and polydispersity index. Then the immunomodulatory activity of LBPs was evaluated through RAW 264.7 cells. The results showed that HWE was the best method to get the highest total sugar and acidic polysaccharides, MAE was preferable to extract polysaccharide-protein complex, but PLE, UAE and HWE could get better immunomodulatory activity polysaccharides than MAE. Besides, the peak 3 in chromatogram of MAE extracted LBPs was obviously higher than those of LBPs produced by other 3 extraction methods, which suggested that peak 1 and peak 2 might be biologically active polysaccharides fractions in LBPs. Therefore, effect of different extraction methods on structure and composition of LBPs attributed to their variance of immunological activities.
已证实多糖具有生物活性，有益于治疗多种疾病。枸杞被广泛用作世界各地的功能性食品，其主要活性成分为枸杞多糖（LBPs）。本研究采用经典热水提取（HWE）、微波辅助提取（MAE）、超声辅助提取（UAE）和加压液体提取（PLE）提取 LBP。根据总多糖含量、糖醛酸含量和蛋白质含量评价 LBPs 的化学性质。采用高效尺寸排阻色谱法结合多角度激光散射和折射率检测器测定分子量、回转半径和多分散指数等特性。然后通过 RAW 264.7 细胞评价 LBPs 的免疫调节活性。结果表明，HWE 是获得最高总糖和酸性多糖的最佳方法，MAE 有利于提取多糖-蛋白质复合物，但 PLE、UAE 和 HWE 比 MAE 能获得更好的免疫调节活性多糖。此外，MAE 提取的 LBPs 图谱中的峰 3 明显高于其他 3 种提取方法得到的 LBPs，提示峰 1 和峰 2 可能是 LBPs 中具有生物活性的多糖部分。因此，不同提取方法对 LBPs 结构和组成的影响归因于其免疫活性的差异。
